ANAPC5 polyclonal, anti-human
€305.00
In stock
SKU
BS61681
Background:
The anaphase-promoting complex (APC) is composed of more than ten subunits, including APC1, APC2, APC4, APC5, APC7, APC8, APC10 and APC11. The APC acts in a cell-cycle dependent manner to promote the separation of sister chromatids during the transition between metaphase and anaphase in mitosis. APC, or cyclosome, accomplishes this progression through the ubiquitination of mitotic cyclins and other regulatory proteins that are targeted for destruction during cell division. APC is phosphorylated, and thus activated, by protein kinases Cdk1/cyclin B and polo-like kinase (Plk). APC is under tight control by a number of regulatory factors, including CDC20, CDH1 and MAD2. Specifically, CDC20 and CDH1 directly bind to and activate APC’s cyclin-ubiquitination activity. In contrast, MAD2 inhibits APC by forming a ternary complex with CDC20 and APC; thus preventing APC activation. APC1, previously referred to as Tsg24 in mice, is the largest of the APC subunits and is encoded by a gene mapping to 2q12.1.
Alternative Name:
Anaphase-promoting complex subunit 5, APC5, Cyclosome subunit 5, ANAPC5, APC5
Application Dilution: WB: 1:500~1:1000
Specificity: ANAPC5 polyclonal antibody detects endogenous levels of ANAPC5 protein.
Immunogen:
Synthetic peptide, corresponding Human ANAPC5.
MW: ~ 85 kDa
Swis Prot.: Q9UJX4
Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Format:
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.3.
Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
For research use only, not for use in diagnostic procedure.
The anaphase-promoting complex (APC) is composed of more than ten subunits, including APC1, APC2, APC4, APC5, APC7, APC8, APC10 and APC11. The APC acts in a cell-cycle dependent manner to promote the separation of sister chromatids during the transition between metaphase and anaphase in mitosis. APC, or cyclosome, accomplishes this progression through the ubiquitination of mitotic cyclins and other regulatory proteins that are targeted for destruction during cell division. APC is phosphorylated, and thus activated, by protein kinases Cdk1/cyclin B and polo-like kinase (Plk). APC is under tight control by a number of regulatory factors, including CDC20, CDH1 and MAD2. Specifically, CDC20 and CDH1 directly bind to and activate APC’s cyclin-ubiquitination activity. In contrast, MAD2 inhibits APC by forming a ternary complex with CDC20 and APC; thus preventing APC activation. APC1, previously referred to as Tsg24 in mice, is the largest of the APC subunits and is encoded by a gene mapping to 2q12.1.
Alternative Name:
Anaphase-promoting complex subunit 5, APC5, Cyclosome subunit 5, ANAPC5, APC5
Application Dilution: WB: 1:500~1:1000
Specificity: ANAPC5 polyclonal antibody detects endogenous levels of ANAPC5 protein.
Immunogen:
Synthetic peptide, corresponding Human ANAPC5.
MW: ~ 85 kDa
Swis Prot.: Q9UJX4
Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Format:
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.3.
Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
For research use only, not for use in diagnostic procedure.
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