Annexin V-Biotin Apoptosis Detection Kit

Annexins are a family of calcium-dependent phospholipid-binding proteins, which bind to phosphatidylserine (PS).

Externalization of phosphatidylserine residues on the outer plasma membrane of apoptotic cells allows detection via Annexin V. Once the apoptotic cells are bound with labelled Annexin V, it can be visualized with fluorescent microscopy or cytometry.

Since loss of membrane integrity is a pathognomonic feature of necrotic cell death, necrotic cells will stain with specific membrane-impermeant nucleic acid dyes such as propidium iodide, the membrane integrity of apoptotic cells can be demonstrated by the exclusion of these dyes.

Advantages & Features
- Easy and one step protocol: get results with few handling steps.
- Detection Method: Flow cytometry (Ex = 488 nm; Em = 530 nm) and fluorescence microscopy.
- Mammalian Reactivity.
- Proven performance for both adherent and suspension cells.
- Versatile: most used kits availables.
- Safe: non-enzymatic assay that avoids fixation.

Includes
- 500 µL Labeled Annexin V-Biotin
- 50 mL Binding Buffer (10x)
- 1 mL Propidium Iodide

Applications
- Detect early/middle stages of Apoptosis.
- Differentiate apoptosis from necrosis.

Shipping & Storage
Shipped in: Gel pack.
Storage: All components should be stored at 4 ºC.
Recommendations: Protect from light and do not freeze.