Anti-DYKDDDDK (FLAG) Red Separopore® 4B Microbeads viewIP™

viewIP™ Anti-DYKDDDDK (FLAG) Red Separopore® Antibody (Monoclonal IgG) (Agarose) - Cat# 20181114

Details
Host: Mouse
Isotype: IgG
Class: Monoclonal
Immunogen: Synthetic peptide corresponding to DYKDDDDK (FLAG)

Application:
Red-colored affinity matrix consists of affinity purified Anti-DYKDDDDK (FLAG) Tag (FG4R) monoclonal antibody covalently attached to red beaded agarose (Separopore®). Suitable for purifying N-terminal, Met-N-terminal, C-terminal DYKDDDDK (FLAG) fusion proteins, 3x DYKDDDDK (FLAG) fusion proteins; greatly reduces the risk of pellet loss. Comparable performance to non-colored affinity gels, but allows the user to easily differentiate pellet from supernatant. This results in greater consistency and more accurate data due to less sample losses. Anti-Flag Red-Separopore® 4B-CL is reliable tool for purifying N-terminal, Met-N-terminal, C-terminal DYKDDDDK (FLAG) fusion proteins, 3x DYKDDDDK (FLAG) fusion proteins.

Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.

Technical Specifications:
Matrix: Separopore® 4B-CL (crosslinked agarose beads, 4%)
Concentration: 25 mg Red Separopore® per mL of buffer
Particle size range: 52 – 165 µm
Ligand coupling method: CNBr activation
Binding capacity: approximately ~600 µg/ml drained gel
Exclusion limit: approximately 2 x 107
pH stability: 3 – 9 (for use), 2 – 10 (for cleaning)
Supplied as 50% suspension in PBS, containing 50% glycerol.
Storage: 4°C

References:
Production, purification, and characterization of scFv TNF ligand fusion proteins. Methods Mol Biol. (2012) 907: 597-609.
Quinolinate phosphoribosyl transferase, a key enzyme in de novo NAD(+) synthesis, suppresses spontaneous cell death by inhibiting overproduction of active-caspase-3. Biochim Biophys Acta. (2010) 1803: 527-33.
Immunopurification of polyribosomal complexes of Arabidopsis for global analysis of gene expression. Plant Physiol. (2005) 138: 624-35.
Identification, molecular cloning, and characterization of a novel GABAA receptor-associated protein, GRIF-1. J Biol Chem. (2002) 277: 30079-90.
Expression and purification of active recombinant ATM protein from transiently transfected mammalian cells. Protein Expr Purif. (2001) 22: 462-6.
S-layer-mediated display of the immunoglobulin G-binding domain of streptococcal protein G on the surface of Caulobacter crescentus: development of an immunoactive reagent. Appl Environ Microbiol. (2007) 73: 3245-53.
Monomers and oligomers of the M2 muscarinic cholinergic receptor purified from Sf9 cells. Biochemistry. (2003) 42: 12960-71.
Expression and characterization of Flag-epitope- and hexahistidine-tagged derivatives of saxiphilin for use in detection and assay of saxitoxin. Toxicon. (2001) 39: 291-301.

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Usage: bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Hazmat Shipping: Non-hazardous
Storage: 2-8°C
Brand Name: bioPLUS™, Separopore®