Anti-SARS-CoV-2 (Nucleocapsid and Spike) Human IgG Flow Test Kit (BT-plex)

The BT-plex™ Human Anti-SARS CoV2 NP and SP Total IgG kit is 2-plex assay panel, using fluorescence-encoded magnetic beads optimized for use with regular flow cytometers. The panel simultaneously detects specific human anti-SARS CoV2 spike protein total IgG and anti-Nucleocapsid protein total IgG in the same sample. The assay panel has been validated for use on serum/plasma and other sample types. The assay panel provides better detection robustness, broader dynamic ranges, and less hands-on time than traditional ELISA methods.

Size: 100 tests

Kit Components: Premixed beads (magnetc), Anti-SARS CoV-2 monoclonal antibody standard cocktail, Human IgG Detection, SAPE, V-bottom 96-well plate

Sample Type: Serum/plasma (1:500), Saliva (1:10)

Protocol time: 3.5 hours

Dynamic range: 6 pg/mL to 25 ng/mL

Limit of detection: Nucleocapsid IgG, 2 pg/mL; Spike Protein IgG, 4 pg/mL

Accuracy: 80-120%

Precision: Intra-assay CV <10%, Inter-assay CV < 15%


Kit Components:
Kit Components Quantity Volume
SARS CoV-2 NP and SP Premixed Beads 1 Bottle 6.0 mL
SARS CoV-2 Antibody Standard (IgG) Cocktail 1 Vial 0.5 mL
Anti-Human IgG1 Detection Antibody 1 Bottle 6.0 mL
BT-plex Streptavidin Phycoerythrin 1 Bottle 6.0 mL
Assay Buffer 2 Bottle 25 mL
20X Wash Buffer 1 Bottle 25 mL
Low-bindingV-bottom Plate 1 Plate NA
Adhesive Plate Sealer 2 Sheets NA
Instruction manual 1