AP2M1 polyclonal, anti-human, mouse, rat

Background:
Adaptins are heterotetrameric subunits of adaptors, which are complexes involved in the formation of Clathrin-coated pits for vesicle-mediated endocytosis. Clathrin and its associated heterotetrameric protein complexes make up the main protein components of the coat surrounding the cytoplasmic face of coated vesicles. The Adaptin family, comprising a, b, b' and g classes, is also responsible for the transport of ligand-receptor complexes from plasma membranes and the trans-Golgi network to lysosomes. Two main types of adaptor proteins (APs), AP-1 and AP-2, are found in Clathrin-coated structures located at the Golgi complex and the plasma membrane of mammalian cells, respectively. Adaptor protein complex 2 (AP-2) is composed of two large Adaptins (a1A/AP2A1 and b1/AP2B1), a medium Adaptin (m2/AP-2m1) and a small Adaptin (s2 long/AP2S1). AP-2m1, a 435 amino acid protein, links Clathrin to receptors in coated vesicles.

Alternative Name:
AP2M1, Adapter-related protein complex 2 mu subunit, Adaptin mu 1, Adaptin-mu2, Adaptor protein complex AP 2 subunit mu, Adaptor protein complex AP-2 subunit mu, Adaptor related protein complex 2 mu 1 subunit, AP 2 mu 2 chain, AP-2 complex subunit mu, AP-2 mu chain, Ap2m1, AP2M1, AP50, CLAPM1, Clathrin adaptor complex AP2 mu subunit, Clathrin assembly protein complex 2 medium chain, Clathrin associated/assembly/adaptor protein medium 1, Clathrin coat adaptor protein AP50, Clathrin coat assembly protein AP50, Clathrin coat associated protein AP50, Clathrin coat-associated protein AP50, HA2 50 kDa subunit, mu2, Plasma membrane adaptor AP-2 50 kDa protein,

Application Dilution:
WB:1:500-1:1,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Specificity: AP2M1 polyclonal antibody detects endogenous levels of AP2M1 protein.

Immunogen:
recombinant protein

MW: 50 kDa

Swiss-Prot: Q96CW1(Human) P84091(Mouse) P84092(Rat)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.