Apaf-1 (clone 2E10), anti-human, mouse, rat

Protein Family: Chaperones and Folding Catalysts

Pathway and Disease: Cell Growth and Death

Description:
Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. The mammalian homologous of the key cell death gene CED-4 in C. elegans was identified recently from human and mouse and designated Apaf1 for apoptosis protease-activating factor 1. Apaf1 binds to cytochrome c (Apaf2) and caspase-9 (Apaf3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis. Apaf1 can also associate with caspase-4 and caspase-8 (4). Apaf1 transcript is ubiquitously expressed in human tissues.

Alternate Names: Apaf-1, Apoptotic protease activating factor 1

Application Notes: E: 1:500-1:1,000; WB: 1:100-1:500; IHC: 1:100-1:500

Accession No.: AAC51678

Antigen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Apaf1.

Format: Each vial contains 0.1 mg IgG in 0.1 ml (1 mg/ml) of PBS pH7.4 with 0.02% sodium azide. Antibody was purified by Protein-A affinity chromatography.

Storage:
Store at 4°C. Minimize freeze-thaw cycles. Product is guaranteed one year from the date of shipment.