APAF1 polyclonal, anti-human, mouse, rat

Quantity: 100 µg

Background:
Apoptotic peptidase activating factor 1, also known as APAF1, is a protein which in humans is encoded by the APAF1 gene. This gene is mapped to chromosome 12q23. It encodes a cytoplasmic protein that initiates apoptosis. And it is an essential downstream effector of p53-mediated apoptosis. This protein contains several copies of the WD40 repeat domain, a caspase recruitment domain(CARD), and an ATPase domain(NB-ARC). In the presence of cytochrome c and dATP, APAF1 assembles into an oligomeric apoptosome, which is responsible for activation of procaspase-9 and maintenance of the enzymatic activity of processed caspase-9. Furthermore, APAF1 is inactivated in metastatic melanomas, leading to defects in the execution of apoptotic cell death. Additionally, APAF1 has been shown to interact with NLRP1, Caspase-9, APIP, BCL2-like 1 and HSPA4.

Description:
Rabbit IgG polyclonal antibody for Apoptotic protease-activating factor 1 (APAF1) detection. Tested with WB, IHC-P in Human, Mouse, Rat.

Synonyms:
APAF 1 antibody, Apaf-1 antibody, APAF_HUMAN antibody, Apaf1 antibody, Apoptotic peptidase activating factor 1 antibody, Apoptotic protease activating factor 1 antibody, Apoptotic protease activating factor antibody, Apoptotic protease-activating factor 1 antibody, CED 4 antibody, CED4 antibody, KIAA0413 antibody

Isotype: Rabbit polyclonal IgG

Immunogen:
A synthetic peptide corresponding to a sequence at the N-terminus of human APAF1(1-19 MDAKARNCLLQHREALEKD), identical to the related mouse sequence, and different from the related rat sequence by one amino acid.A synthetic peptide corresponding to a sequence at the N-terminus of human APAF1(1-19 MDAKARNCLLQHREALEKD), identical to the related mouse sequence, and different from the related rat sequence by one amino acid.

Form: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Specificity:
No cross reactivity with other proteins.

Reactivity: Reacts with: human, rat .
Predicted to work with: mouse.

Applications: WB, IHC-P

Reconstitution:
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage: At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing.