APAF1 Polyclonal Antibody

Background:
This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms.

Research Area: Cancer, Cell Biology, Metabolism

Synonyms:
APAF 1, Apaf-1, APAF, Apaf1, Apoptotic peptidase activating factor 1, Apoptotic protease activating factor 1, Apoptotic protease activating factor, Apoptotic protease-activating factor 1, CED 4, CED4, KIAA0413

Immunogen: Synthetic peptide of human APAF1

Swissprot: O14727
Gene Accession: NP_863659

Calculated MW: 142 kDa

Purification Method: Affinity purification

Buffer: PBS with 0.05% sodium azide and 50% glycerol, PH7.4

Concentration: 0.7 mg/mL
Dilution: WB 1:500-1:2000, IHC 1:50-1:200

Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Alternative for product with catalog number:
sc-7231/sc-26685/sc-7232/sc-33870/sc-33871/sc-65890/sc-65891/sc-65890/sc-65891/sc-8339