APAF1 Polyclonal Antibody

Background:
This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms.

Research Area: Cancer, Cell Biology, Metabolism

Synonyms:
APAF1, APAF-1, CED4

Immunogen: Recombinant fusion protein of human APAF1 (NP_863651.1).

Swissprot: O14727
Gene ID: 317

Calculated MW: 37 kDa/133 kDa/135 kDa/137 kDa/140 kDa/141 kDa
Observed MW: 142 kDa

Purification Method: Affinity purification

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3

Concentration: 1 mg/mL
Dilution: "WB 1:500-1:2000"

Storage:
Store at -20°C. Avoid freeze / thaw cycles.