Arginine Separopore® 6B-CL

Application: Arginine-Separopore® 6B is used for purification of molecules with biospecific or charge dependent affinity for Arginine. It is also used for the purification of lysine-binding proteins from serum and to purify plasminogen, plasminogen activator, ribosomal RNA, and double-stranded DNA.

Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.

Specifications:
Ligand: Arginine
Particle size range: 52 – 180 µm
Molecular weight range: 6x104 - 2x107
Matrix: Separopore® 6B (agarose beads, 6%)
Matrix active group: Epoxy
Attachment: α-Amino
Extent of coupling: ~4 μmol / ml
pH stability: 4 – 9
Flow Specifications: 70 – 140 cm / h
Supplied as a suspension in 2M NaCl, 0.02% thimerosal
Storage temp.: 2 – 8 °C

References:
Expression analysis and purification of human recombinant tissue type plasminogen activator (rt-PA) from transgenic tobacco plants. Prep Biochem Biotechnol. (2011) 41: 175-86.
Simplified recombinant plasmin: production and functional comparison of a novel thrombolytic molecule with plasma-derived plasmin. Thromb Haemost. (2008) 100: 413-9.
Glycosylation variant analysis of recombinant human tissue plasminogen activator produced in urea-cycle-enzyme-expressing Chinese hamster ovary (CHO) cell line. J Biosci Bioeng. (2006) 102: 447-51.
Baboon lipoprotein(a) binds very weakly to lysine-agarose and fibrin despite the presence of a strong lysine-binding site in apolipoprotein(a) kringle IV type 10. Biochemistry. (2005) 44: 555-64.
Anti-angiogenic role of angiostatin during corneal wound healing. Exp Eye Res. (2004) 78: 579-89.
Angiostatin and matrix metalloprotease expression following ischemic acute renal failure. Am J Physiol Renal Physiol. (2004) 286: F893-902.
A new method of purifying fibrinogen with both biological and immunological activity from human plasma. Prep Biochem Biotechnol. (2003) 33: 239-52.
Generation and role of angiostatin in human platelets. Blood. (2003) 102: 3217-23.
Identification of a novel apolipoprotein(a)-related protein from the European hedgehog (Erinaceus europaeus). DNA Seq. (2003) 14: 15-23.
Malignant ascites fluid (MAF), including ovarian-cancer-associated MAF, contains angiostatin and other factor(s) which inhibit angiogenesis. Gynecol Oncol. (2002) 86: 279-87.
Angiostatin II is the predominant glycoform in pleural effusates of rabbit VX-2 lung tumors. J Lab Clin Med. (2002) 139: 316-23.
Antifibrinolytic effect of single apo(a) kringle domains: relationship to fibrinogen binding. Protein Eng. (2001) 14: 427-38.
Purification and partial characterisation of alpha(2)-antiplasmin and plasmin(ogen) from ostrich plasma. Comp Biochem Physiol B Biochem Mol Biol. (2001) 129: 809-20.
Identification of calnexin as a binding protein for Amadori-modified glycated albumin. Biochem Biophys Res Commun. (2001) 284: 602-6.
Properties of human free apolipoprotein(a) and lipoprotein(a) after either freezing or lyophilization in the presence and absence of cryopreservatives. Anal Biochem. (2001) 288: 201-8.

Related products:
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L-Glutamic Acid Separopore® 4B-CL (SKU # 20181068)
Glycine Separopore® 4B-CL (SKU # 20181065)
L-Histidine Separopore® 4B-CL (SKU # 20181069)
L-Lysine Separopore® 4B-CL (SKU # 20181070)
L-Phenylalanine Separopore® 4B-CL (SKU # 20181071)
Poly-L-Lysine Separopore® 4B-CL (SKU # 20181075)
Glutathione Separopore® 4B (SKU # 20181050)

Usage: bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Hazmat Shipping: Non-Hazardous
Storage: 2-8°C