Aspartate Aminotransferase (AST/GOT) Activity Assay Kit (Reitman-Frankel Method)

Detection Principle:
AST/GOT enables alpha-ketoglutaric acid and aspartic acid to displace amino and keto groups to form glutamic acid and oxaloacetic acid. Oxaloacetic acid can decarboxylate itself to form Pyroracemic acid during the reaction. Pyroracemic acid reacted with 2,4-dinitrophenylhydrazine(DNPH) to form 2,4, dinitrophenylhydrazone showing reddish brown in alkaline solution. Measure the OD values and calculate the enzyme activity.
Instrument: Microplate reader
Assay Time: 80 min

Tested Sample Types: Serum,plasma,animal tissue,cells,cell culture supernatant

Research Area's: Enzymes,Liver Biomarkers

Shelf Life: 6 Months

Storage:
2-8℃

Citations:


Detection Principle: https://www.sciencedirect.com/science/article/pii/S2213713018300014
https://works.bepress.com/dr_hesham_mustafa/26/download/
https://www.ncbi.nlm.nih.gov/pubmed/31824338
https://iopscience.iop.org/article/10.1088/1742-6596/1374/1/012009/pdf
https://pubs.acs.org/doi/abs/10.1021/acsabm.0c00841
https://pubmed.ncbi.nlm.nih.gov/32678475/
https://pubmed.ncbi.nlm.nih.gov/32705206/
https://pubmed.ncbi.nlm.nih.gov/33022138/
https://onlinelibrary.wiley.com/doi/10.1002/mnfr.202001133