Asymmetric DiMethyl-Histone H4-R3 Polyclonal Antibody

Background:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy.

Research Area: Cancer, Epigenetics and Nuclear Signaling

Synonyms:
FO108, H4, H4/n, H4F2, H4FN, HIST2H4, Histone H4, HIST1H4A, HIST2H4A

Immunogen: A synthetic methylated peptide corresponding to residues surrounding Arg3 of human histone H4

Swissprot: P62805
Gene ID: 8370

Calculated MW: 11 kDa
Observed MW: 13 kDa

Purification Method: Affinity purification

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3

Concentration: 1 mg/mL
Dilution: "WB 1:500-1:2000 IHC 1:50-1:200 IF 1:50-1:200"

Storage:
Store at -20°C. Avoid freeze / thaw cycles.