ATF2 (Phospho-T71) polyclonal, anti-human

Background:
Eukaryotic gene transcription is regulated by sequence-specific transcription factors which bind modular cis-acting promotor and enhancer elements. The ATF/CREB transcription factor family binds the palindromic cAMP response element (CRE) octanucleotide TGACGTCA. The ATF/CREB family includes CREB-1, CREB-2 (also designated ATF-4), ATF-1, ATF-2 and ATF-3. This family of proteins contain highly divergent N-terminal domains, but share a C-terminal leucine zipper for dimerization and DNA binding. ATF-2 forms homodimers and heterodimers with c-Jun to initiate CRE-dependent transcription. Phosphorylation of ATF-2 at Thr 69 and Thr 71 by stress-activated kinases is necessary for transcriptional activation. Myc also induces phosphorylation of ATF-2 at Thr 69 and Thr 71 to prolong the half-life of ATF-2. ATF-2 also functions as a histone acetyltransferase (HAT) by specifically acetylating histones H2B and H4 in vitro. The gene encoding human ATF-2 maps to chromosome 2q31.1.

Alternative Name:
LRP-1, A2MR, APOER, CD91, Activating transcription factor 2, Activating transcription factor 2 splice variant ATF2 var2, ATF 2, Atf-2, Atf2, ATF2 protein, ATF2, cAMP Response Element Binding Protein 2, cAMP response element binding protein CRE BP1, cAMP response element-binding protein CRE-BP1, cAMP responsive element binding protein 2, formerly, cAMP-dependent transcription factor ATF-2, cAMP-responsive element-binding protein 2, CRE BP1, CRE-BP, CREB 2, CREB-2, CREB2, CREBP1, Cyclic AMP dependent transcription factor ATF 2, Cyclic AMP-dependent transcription factor ATF-2, Cyclic AMP-responsive element-binding protein 2, D130078H02Rik, D18875, HB 16, HB16, Histone acetyltransferase ATF2, MGC105211, MGC105222, MGC111558, MGC142504, mXBP, MXBP protein, Tg(Gzma-Klra1)7Wum, TREB 7, TREB7,

Application Dilution:
WB:1:1,000
ICC:1:50-1:200

Specificity: ATF2 (Phospho-T71) polyclonal antibody detects endogenous levels of ATF2 protein only when phosphorylated at T71.

Immunogen:
Synthetic phospho-peptide corresponding to residues surrounding Thr71 of human ATF2

MW: 55 kDa

Swiss-Prot: Q07954(Human) Q91ZX7(Mouse) Unigene:22436(Rat)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.