β-lactamases + Colistin resistance lyophilised RTU qPCR CE/IVD

Intended Use
Vitassay qPCR β-lactamases + Colistin resistance allows the qualitative detection and identification of the main β-lactamases-resistant genes: clúster blaCTX-M-A (blaCTX-M-1 and blaCTX-M-9), clúster blaCTX-M-B (blaCTX-M-2, blaCTX-M-8 y blaCTX-M-25), blaTEM, blaSHV and the colistin resistance coding gene, mcr-1, in clinical samples. This product is intended to facilitate the diagnosis and surveillance of infections caused by antibiotic-resistance and colonization, together with the patient's clinical data, epidemiological risk factors, and the results of other laboratory tests.

Vitassay qPCR Kits contains in each well enzymes, buffer, primer and probes as well as an internal control in a lyophilized format.
The kits include a positive and a negative control.

Identification of pathogen DNA or RNA occurs by the use of target specific primers ans fluorecent labeled hydrolysis probes which hybridize conserved regions in the genomes of the different pathogens.

Kit Content:
- β-lactamases + Colistin resistance strips low/high profile: 4x8-well strip containg lyophilised target primer, polymerase (and reverse transciptase) and nucleotides
- β-lactamases + Colistin resistance Positive Control (red vial): 1 vial
- PCR grade water (white vial): 1 vial
- Resuspension Buffer (green vial): 1 vial x1,8 mL
- Negative control (yellow):1 vial x 1,8 mL
- Optical caps: 4x8 cap strip

Kit Format:
qualitative