B-Raf (Phospho-T401) polyclonal, anti-human, mouse, rat

Background:
Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk) and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis, while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. Raf-A, a second member of the Raf gene family of serine/threonine protein kinases, exhibits substantial homology to Raf-1 within the kinase domain of the two molecules, but less homology elsewhere. Expression of Raf-B is highly restricted, with highest levels in the cerebrum and testis.

Alternative Name:
BRAF, FLJ95109, 94 kDa B raf protein, B raf 1, B Raf proto oncogene serine threonine protein kinase, B Raf proto oncogene, serine/threonine kinase, B RAF1, B-Raf proto-oncogene serine/threonine-protein kinase (p94), BRAF 1, BRAF, BRAF, BRAF1, cRmil, MGC126806, MGC138284, Murine sarcoma viral (v-raf) oncogene homolog B1, Murine sarcoma viral v raf oncogene homolog B1, NS7, Oncogen BRAF, oncogene BRAF1, p94, Proto-oncogene B-Raf, Proto-oncogene c-Rmil, RAFB 1, RAFB1, RMIL, Serine/threonine-protein kinase B-raf, v raf murine sarcoma viral oncogene homolog B, v raf murine sarcoma viral oncogene homolog B1, v-Raf murine sarcoma viral oncogene homolog B1,

Application Dilution:
WB:1:1,000-1:5,000
ICC:1:100-1:500

Specificity: B-Raf (Phospho-T401) polyclonal antibody detects endogenous levels of B-Raf protein only when phosphorylated at T401.

Immunogen:
Synthetic phospho-peptide corresponding to residues surrounding Thr401 of human B Raf.

MW: 84 kDa

Swiss-Prot: P15056(Human) P28028(Mouse) EntrezGene:114486(Rat)

Purification & Purity:
ProA affinity purified

Format:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage:
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

For research use only, not for use in diagnostic procedure.