B-Raf (Phospho-Thr753) polyclonal, anti-human, mouse, rat

Specificity:
B-Raf (Phospho-Thr753) Antibody detects endogenous levels of BRAF1 only when phosphorylated at Thr753.

Synonyms: B-RAF, B-RAF proto-oncogene serine/threonine-protein kinase, B-Raf proto-oncogene serine/threonine-protein kinase, BRAF, BRAF1, c-RMIL, kinase B-Raf, p94, RAFB1, RMIL, RMIL serine/threonine-protein kinase, v-Raf murine sarcoma viral oncogene homolog B1

Immunogen:
The antiserum was produced against synthesized phospho-peptide derived from human B-Raf around the phosphorylation site of Thr753.

Immunogen Sequence:
Phospho

Modification Sites: H:S753, M:S790, R:S790

MW (kDa): 84 kDa

NCBI Gene Symbol: BRAF1

Database Links:
Gene ID: 673
Swiss Prot No.: P15056
OMIM No.: 114500/115150/164757/211980/605027
Unigene No.: Hs.550061

Application Dilutions: WB: 1:500~1:1000, ELISA: 1:10000

Purification:
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Physical Format:
Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Concentration: 1 mg/ml

Storage: Stable at -20°C for at least 1 year.