BCA Protein Colorimetric Assay Kit

Detection Principle:
Cu2+ can be reduced to Cu+ by protein in alkaline condition. Cu+ can combine with BCA reagent and form purple complex, which has a maximum absorption peak at 562 nm. The absorbance value is proportional to the protein concentration. Therefore, the protein concentration can be calculated according to the OD value.
Instrument: Microplate reader
Assay Time: 40 min

Tested Sample Types: Serum,plasma,cell culture supernatant,tissue,cells

Research Area's: Amino acids & proteins

Shelf Life: 12 Months

Storage:
RT

Citations:


Detection Principle: https://www.sciencedirect.com/science/article/pii/S0014299919301967
https://europepmc.org/abstract/med/29518790
https://www.sciencedirect.com/science/article/abs/pii/S0304394018302866
https://www.ncbi.nlm.nih.gov/pubmed/31824338
https://pubmed.ncbi.nlm.nih.gov/32373979/
https://www.researchgate.net/profile/Olatunde_Akanbi/publication/338698785
https://www.frontiersin.org/articles/10.3389/fnins.2020.00659/pdf
https://www.scielo.br/scielo.php?pid=S0102-76382020005005206&script=sci_arttext
https://pubmed.ncbi.nlm.nih.gov/32466476/