Beclin-1 (Ser15) polyclonal, anti-human, mouse
€462.00
In stock
SKU
254515
Catalog Nr.: 254515
Size: 0.1 ml
Isotype: Rabbit Ig
Applications: E, WB, IP
Size: 0.1 ml
Isotype: Rabbit Ig
Applications: E, WB, IP
Protein Family: Chaperones and Folding Catalysts
Pathway and Disease: Cancers, Cell Growth and Death, Folding, Sorting and Degradation, Infectious Diseases, Signaling Molecules and Interaction
Description:
Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. Beclin-1, a coiled-coil Bcl-2-interacting protein homologous to the yeast autophagy gene apg6, is a mammalian autophagy gene that can inhibit tumorigenesis and is expressed at reduced levels in human breast carcinoma, suggesting that defects in autophagy proteins may contribute to the development or progression of tumors. Bcl-2 can bind to Beclin-1 and inhibit Beclin-1-dependent autophagy in yeast and mammalian cells, suggesting that Bcl-2 functions as an anti-autophagy protein as well as an anti-apoptotic protein, which helps maintain autophagy at levels that are more compatible with cell survival rather than cell death. ULK1 protein kinase and the VPS34 lipid kinase are key regulators of autophagy initiation and progression. Activated during low nutrient conditions, ULK1 directly phosphorylates Beclin-1 at multiple sites including Ser15.
Alternate Names: Beclin-1 , Coiled-coil myosin-like Bcl-2-interacting protein, Protein GT197, BECN1
Application Notes: Beclin-1 (Ser15) Antibody recognizes endogenous and transfected levels of Beclin-1 only when phosphorylated at Ser15 (Ser14 in mouse). Low endogenous expression levels are at the detection limit of this antibody. E: 1:500-1:2,000; WB: 1:100-1:1,000; IP: 1:100-1:200
Accession No.: Q14457
Antigen: KLH-conjugated synthetic phosphopeptide encompassing a sequence surrounding Ser15 of human beclin-1.
Format: Each vial contains 0.1 ml IgG in PBS pH7.4, 0.1% BSA and 20% glycerol. Antibody was purified by immunogen affinity chromatography.
Storage:
Store at -20°C. Minimize freeze-thaw cycles. Product is guaranteed 6 months from the date of shipment.
Product Citations:
[1] Hwang J-Y et al. 2017. Cell Death Differ. PMID 27935582.
[2] Miki Y et al. 2016. Brain Pathol. PMID 26260450.
[3] Chauhan S et al. 2015. Mol Cell. PMID 25891078.
[4] Egan DF et al. 2015. Mol Cell. PMID 26118643.
[5] Tanji K. et al. 2015 Biochem Biophys Res Commun. PMID 26299928.
[6] Russell R. et al. 2013. Nat Cell Biol. 15(7): 741-50. PMID 23685627.
Pathway and Disease: Cancers, Cell Growth and Death, Folding, Sorting and Degradation, Infectious Diseases, Signaling Molecules and Interaction
Description:
Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. Beclin-1, a coiled-coil Bcl-2-interacting protein homologous to the yeast autophagy gene apg6, is a mammalian autophagy gene that can inhibit tumorigenesis and is expressed at reduced levels in human breast carcinoma, suggesting that defects in autophagy proteins may contribute to the development or progression of tumors. Bcl-2 can bind to Beclin-1 and inhibit Beclin-1-dependent autophagy in yeast and mammalian cells, suggesting that Bcl-2 functions as an anti-autophagy protein as well as an anti-apoptotic protein, which helps maintain autophagy at levels that are more compatible with cell survival rather than cell death. ULK1 protein kinase and the VPS34 lipid kinase are key regulators of autophagy initiation and progression. Activated during low nutrient conditions, ULK1 directly phosphorylates Beclin-1 at multiple sites including Ser15.
Alternate Names: Beclin-1 , Coiled-coil myosin-like Bcl-2-interacting protein, Protein GT197, BECN1
Application Notes: Beclin-1 (Ser15) Antibody recognizes endogenous and transfected levels of Beclin-1 only when phosphorylated at Ser15 (Ser14 in mouse). Low endogenous expression levels are at the detection limit of this antibody. E: 1:500-1:2,000; WB: 1:100-1:1,000; IP: 1:100-1:200
Accession No.: Q14457
Antigen: KLH-conjugated synthetic phosphopeptide encompassing a sequence surrounding Ser15 of human beclin-1.
Format: Each vial contains 0.1 ml IgG in PBS pH7.4, 0.1% BSA and 20% glycerol. Antibody was purified by immunogen affinity chromatography.
Storage:
Store at -20°C. Minimize freeze-thaw cycles. Product is guaranteed 6 months from the date of shipment.
Product Citations:
[1] Hwang J-Y et al. 2017. Cell Death Differ. PMID 27935582.
[2] Miki Y et al. 2016. Brain Pathol. PMID 26260450.
[3] Chauhan S et al. 2015. Mol Cell. PMID 25891078.
[4] Egan DF et al. 2015. Mol Cell. PMID 26118643.
[5] Tanji K. et al. 2015 Biochem Biophys Res Commun. PMID 26299928.
[6] Russell R. et al. 2013. Nat Cell Biol. 15(7): 741-50. PMID 23685627.
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