Benzamidine Separopore® 6B
€0.00
In stock
SKU
BW-20181111
Application:
Benzamidine-Separopore® 6B is p-aminobenzamidine covalently attached to Separopore® 6B by the epoxy coupling method. p-Aminobenzamidine (PAB), is an inhibitor of trypsin-like serine protease. Trypsin and trypsin-like serine proteases bind to Benzamidine-Separopore® 6B and can thus be used for purification and/or removal of these substances.
Benzamidine-Separopore® 6B can be used for purifying serine proteases such as trypsin, bovine thrombin, urokinase, human enterokinase, acrosin, native plasminogen, kallikrein, prekallikrein, collagenase and clostripain. For recombinant purification, Benzamidine-Separopore® 6B can be used for removal of serine proteases such as thrombin and enterokinase after cleavage of purification tags.
Benzamiding-Separopore® 6B is tested for its binding capacity for trypsin, but the resin has been used in the purification of a variety of proteins including thrombin, Adenylyl cyclase-activating protease from bovine sperm, Fibrinolytic enzyme from Agkistrodon contortrix, Tryptase from rat skin,
Serine endopeptidase from rat mammary tissue, Glycosylation-enhancing factor (GEF), Plasminogen activator from embryo lung culture, Plasminogen activator from human umbilical vein endothelial cells and Thrombin-like enzyme from Bothrops atrox venom
Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.
Technical Specifications:
Ligand: p-Aminobenzamidine (PAB)
Matrix: Separopore® 6B (agarose beads, 6%)
Particle size range: 52 – 165 µm
Matrix activation: Epoxy activation
Ligand density: 7 μmole p-aminobenzamidine/ml drained gel
Binding capacity: > 10mg trypsin/ml drained gel
Exclusion limit: approximately 2 x 107
pH stability: Long term: 3 – 11; Short term: 2 – 13
Chemical stability: Stable to all commonly used aqueous buffers
Physical stability: Negligible volume variation due to changes in pH or ionic strength
Storage temp: 4 –8 °C
Benzamidine-Separopore® 6B is supplied as pre-swollen gel in 20% ethanol
References:
Expression and purification of recombinant rabbit factor VII. Thromb Res. (2000) 98: 203-11.
Purification of a second kallikrein from bovine pancreas. Prep Biochem. (1993) 23: 321-31.
Purification and properties of a single-chain urokinase-type plasminogen activator form produced by subcultured human umbilical vein endothelial cells. J Biol Chem. (1988) 263: 15139-45.
Arginyl-binding sites of human plasminogen. Thromb Res. (1986) 41: 689-98.
Isolation and characterization of bovine plasma prekallikrein (Fletcher factor). Biochemistry. (1979) 18: 5743-50.
Characterization of bovine factor XIIa (activated Hageman factor). Biochemistry. (1977) 16: 4182-8.
Isolation and characterization of bovine factor XII (Hageman factor). Biochemistry. (1977) 16: 2270-8.
Isolation and characterization of bovine factor VII. Biochemistry. (1975) 14: 4928-34.
The prufication of bovine thrombin by affinity chromatography on benzamidine-agarose. Hoppe Seylers Z Physiol Chem. (1972) 353: 810-4.
Production and purification of TAL6003: A novel version of the protease plasmin. Protein Expr Purif. (2012) 88: 41-46.
Biochemical characterization and comparative analysis of two distinct serine proteases from Bothrops pirajai snake venom. Biochimie. (2012) 94: 2545-58.
Simple preparation of pacific cod trypsin for enzymatic Peptide synthesis. J Amino Acids. (2011) 2011: 912382.
Microscale affinity purification of trypsin reduces background peptides in matrix-assisted laser desorption/ionization mass spectrometry of protein digests. J Proteomics. (2011) 74: 948-57.
High-level production and purification of clostripain expressed in a virulence-attenuated strain of Clostridium perfringens. Protein Expr Purif. (2011) 76: 83-9.
CCSV-MPase, a novel procoagulant metalloproteinase from Cerastes cerastes venom: purification, biochemical characterization and protein identification. Protein J. (2010) 29: 466-74.
Functional and structural characterization of a new thrombin-like enzyme from Bothrops alternatus snake venom. Toxicon. (2010) 55: 1365-77.
Metal dependent hydrolysis of β-casein by sIgA antibodies from human milk. J Mol Recognit. (2011) 24: 45-59.
Structural and functional properties of Bp-LAAO, a new L-amino acid oxidase isolated from Bothrops pauloensis snake venom. Biochimie. (2009) 91: 490-501.
Identification of a serine protease as a major allergen (Per a 10) of Periplaneta americana. Allergy. (2008) 63: 768-76.
Antitumoural effect of an L-amino acid oxidase isolated from Bothrops jararaca snake venom. Basic Clin Pharmacol Toxicol. (2008) 102: 533-42.
Hemorrhagic, coagulant and fibrino(geno)lytic activities of crude venom and fractions from mapanare (Bothrops colombiensis) snakes. Comp Biochem Physiol C Toxicol Pharmacol. (2008) 147: 113-21.
Purification, characterization, and cDNA cloning of a myofibril-bound serine proteinase from the skeletal muscle of crucian carp (Carassius auratus). J Agric Food Chem. (2007) 55: 1510-6.
Recovery of urokinase from integrated mammalian cell culture cryogel bioreactor and purification of the enzyme using p-aminobenzamidine affinity chromatography. J Mol Recognit. (2006) 19: 332-9.
A novel type of myofibril-bound serine protease from white croaker (Argyrosomus argentatus). Comp Biochem Physiol B Biochem Mol Biol. (2005) 141: 231-6.
Quantitative determination of the ligand content in Benzamidine Sepharose 4 Fast Flow media with ion-pair chromatography. J Chromatogr A. (2005) 1070: 103-9.
Partial purification and characterization of pro-phospholipase A2 activating proteases from gill membranes of the red sea bream, Chrysophrys major. Comp Biochem Physiol B Biochem Mol Biol. (2005) 141: 121-7.
Purification and characterization of jararassin-I, A thrombin-like enzyme from Bothrops jararaca snake venom. Acta Biochim Biophys Sin (Shanghai). (2004) 36: 798-802.
Preparation of recombinant alpha-thrombin: high-level expression of recombinant human prethrombin-2 and its activation by recombinant ecarin. J Biochem. (2004) 135: 577-82.
Purification and characterization of the fusion protein trypsin-streptavidin expressed in Escherichia coli. J Protein Chem. (2002) 21: 413-8.
Purification, characterization and crystallization of Jararacussin-I, a fibrinogen-clotting enzyme isolated from the venom of Bothrops jararacussu. Toxicon. (2002) 40: 1307-12.
Localization of a proteolytic enzyme within the efferent and deferent duct epithelial cells of the turkey (Meleagris gallopavo) using immunohistochemistry. Biol Reprod. (2002) 67: 276-81.
Xanthine oxidase and aldehyde oxidase: a simple procedure for the simultaneous purification from rat liver. Arch Biochem Biophys. (2002) 400: 48-53.
Construction and characterization of trifunctional single-chain urokinase-type plasminogen activators. Eur J Biochem. (2002) 269: 1708-13.
Construction and characterization of a recombinant chimeric plasminogen activator consisting of a fibrin peptide and a low molecular mass single-chain urokinase. Biochimie. (2001) 83: 1049-55.
Plasmin induces local thrombolysis without causing hemorrhage: a comparison with tissue plasminogen activator in the rabbit. Thromb Haemost. (2001) 86: 739-45.
Binding of plasminogen and tissue plasminogen activator to plasmin-modulated factor X and factor Xa. Biochemistry. (2001) 40: 6293-302.
Identification of a myofibril-bound serine proteinase (MBSP) in the skeletal muscle of lizard fish Saurida wanieso which specifically cleaves the arginine site. Comp Biochem Physiol B Biochem Mol Biol. (2000) 125: 255-64.
Purification and characterization of a trypsin-like serine proteinase from rat brain slices that degrades laminin and type IV collagen and stimulates protease-activated receptor-2. J Neurochem. (2000) 74: 1731-8.
Lactogenic hormones regulate xanthine oxidoreductase and beta-casein levels in mammary epithelial cells by distinct mechanisms. Arch Biochem Biophys. (2000) 373: 318-27.
Mouse mammary gland xanthine oxidoreductase: purification, characterization, and regulation. Arch Biochem Biophys. (1999) 371: 308-16.
Protease dependent activation of endothelial cells by peritoneal dialysis effluents. Thromb Haemost. (1999) 82: 1334-41.
Purification of mast cell proteases from murine skin. Exp Dermatol. (1999) 8: 413-8.
Spectrum of extracellular matrix degrading enzymes in normal and malignant T lymphocytes. Anticancer Res. (1999) 19: 1945-52.
Purification and further characterization of enteropeptidase from porcine duodenum. J Biochem. (1999) 125: 947-51.
Isolation and characterization of two digestive trypsin-like proteinases from larvae of the stalk corn borer, Sesamia nonagrioides. Insect Biochem Mol Biol. (1999) 29: 177-84.
Not more than three tissue kallikreins identified from organs of the guinea pig. Biol Chem. (1999) 380: 63-73.
Purification, characterization, and cloning of enamel matrix serine proteinase 1. J Dent Res. (1998) 77: 377-86.
Identification and activation of profollipsin, a latent precursor form of porcine follipsin. Eur J Biochem. (1997) 245: 392-7.
Purification and characterization of hepatic aldehyde oxidase in male and female mice. Arch Biochem Biophys. (1997) 338: 29-34.
Purification and characterization of hepsin from rat liver microsomes. Biochim Biophys Acta. (1997) 1337: 85-95.
Isolation and some molecular properties of a trypsin-like enzyme from larvae of European corn borer Ostrinia nubilalis Hübner (Lepidoptera: Pyralidae). Insect Biochem Mol Biol. (1996) 26: 883-9.
Purification of rat liver xanthine oxidase and xanthine dehydrogenase by affinity chromatography on benzamidine-sepharose. Arch Biochem Biophys. (1996) 332: 135-41.
Early trypsin, a female-specific midgut protease in Aedes aegypti: isolation, aminoterminal sequence determination, and cloning and sequencing of the gene. Insect Biochem Mol Biol. (1996) 26: 119-26.
Alpha 1-antitrypsin is degraded and non-functional in chronic wounds but intact and functional in acute wounds: the inhibitor protects fibronectin from degradation by chronic wound fluid enzymes. J Invest Dermatol. (1995) 105: 572-8.
Related products:
Benzamidine-Separopore® 6B (Columns) (SKU # 20170024)
Usage: bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product, food additive or as a household chemical
Hazmat Shipping : Non-hazardous
Storage : 2-8°C
Brand Name : bioPLUS™, Separopore®
Benzamidine-Separopore® 6B is p-aminobenzamidine covalently attached to Separopore® 6B by the epoxy coupling method. p-Aminobenzamidine (PAB), is an inhibitor of trypsin-like serine protease. Trypsin and trypsin-like serine proteases bind to Benzamidine-Separopore® 6B and can thus be used for purification and/or removal of these substances.
Benzamidine-Separopore® 6B can be used for purifying serine proteases such as trypsin, bovine thrombin, urokinase, human enterokinase, acrosin, native plasminogen, kallikrein, prekallikrein, collagenase and clostripain. For recombinant purification, Benzamidine-Separopore® 6B can be used for removal of serine proteases such as thrombin and enterokinase after cleavage of purification tags.
Benzamiding-Separopore® 6B is tested for its binding capacity for trypsin, but the resin has been used in the purification of a variety of proteins including thrombin, Adenylyl cyclase-activating protease from bovine sperm, Fibrinolytic enzyme from Agkistrodon contortrix, Tryptase from rat skin,
Serine endopeptidase from rat mammary tissue, Glycosylation-enhancing factor (GEF), Plasminogen activator from embryo lung culture, Plasminogen activator from human umbilical vein endothelial cells and Thrombin-like enzyme from Bothrops atrox venom
Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.
Technical Specifications:
Ligand: p-Aminobenzamidine (PAB)
Matrix: Separopore® 6B (agarose beads, 6%)
Particle size range: 52 – 165 µm
Matrix activation: Epoxy activation
Ligand density: 7 μmole p-aminobenzamidine/ml drained gel
Binding capacity: > 10mg trypsin/ml drained gel
Exclusion limit: approximately 2 x 107
pH stability: Long term: 3 – 11; Short term: 2 – 13
Chemical stability: Stable to all commonly used aqueous buffers
Physical stability: Negligible volume variation due to changes in pH or ionic strength
Storage temp: 4 –8 °C
Benzamidine-Separopore® 6B is supplied as pre-swollen gel in 20% ethanol
References:
Expression and purification of recombinant rabbit factor VII. Thromb Res. (2000) 98: 203-11.
Purification of a second kallikrein from bovine pancreas. Prep Biochem. (1993) 23: 321-31.
Purification and properties of a single-chain urokinase-type plasminogen activator form produced by subcultured human umbilical vein endothelial cells. J Biol Chem. (1988) 263: 15139-45.
Arginyl-binding sites of human plasminogen. Thromb Res. (1986) 41: 689-98.
Isolation and characterization of bovine plasma prekallikrein (Fletcher factor). Biochemistry. (1979) 18: 5743-50.
Characterization of bovine factor XIIa (activated Hageman factor). Biochemistry. (1977) 16: 4182-8.
Isolation and characterization of bovine factor XII (Hageman factor). Biochemistry. (1977) 16: 2270-8.
Isolation and characterization of bovine factor VII. Biochemistry. (1975) 14: 4928-34.
The prufication of bovine thrombin by affinity chromatography on benzamidine-agarose. Hoppe Seylers Z Physiol Chem. (1972) 353: 810-4.
Production and purification of TAL6003: A novel version of the protease plasmin. Protein Expr Purif. (2012) 88: 41-46.
Biochemical characterization and comparative analysis of two distinct serine proteases from Bothrops pirajai snake venom. Biochimie. (2012) 94: 2545-58.
Simple preparation of pacific cod trypsin for enzymatic Peptide synthesis. J Amino Acids. (2011) 2011: 912382.
Microscale affinity purification of trypsin reduces background peptides in matrix-assisted laser desorption/ionization mass spectrometry of protein digests. J Proteomics. (2011) 74: 948-57.
High-level production and purification of clostripain expressed in a virulence-attenuated strain of Clostridium perfringens. Protein Expr Purif. (2011) 76: 83-9.
CCSV-MPase, a novel procoagulant metalloproteinase from Cerastes cerastes venom: purification, biochemical characterization and protein identification. Protein J. (2010) 29: 466-74.
Functional and structural characterization of a new thrombin-like enzyme from Bothrops alternatus snake venom. Toxicon. (2010) 55: 1365-77.
Metal dependent hydrolysis of β-casein by sIgA antibodies from human milk. J Mol Recognit. (2011) 24: 45-59.
Structural and functional properties of Bp-LAAO, a new L-amino acid oxidase isolated from Bothrops pauloensis snake venom. Biochimie. (2009) 91: 490-501.
Identification of a serine protease as a major allergen (Per a 10) of Periplaneta americana. Allergy. (2008) 63: 768-76.
Antitumoural effect of an L-amino acid oxidase isolated from Bothrops jararaca snake venom. Basic Clin Pharmacol Toxicol. (2008) 102: 533-42.
Hemorrhagic, coagulant and fibrino(geno)lytic activities of crude venom and fractions from mapanare (Bothrops colombiensis) snakes. Comp Biochem Physiol C Toxicol Pharmacol. (2008) 147: 113-21.
Purification, characterization, and cDNA cloning of a myofibril-bound serine proteinase from the skeletal muscle of crucian carp (Carassius auratus). J Agric Food Chem. (2007) 55: 1510-6.
Recovery of urokinase from integrated mammalian cell culture cryogel bioreactor and purification of the enzyme using p-aminobenzamidine affinity chromatography. J Mol Recognit. (2006) 19: 332-9.
A novel type of myofibril-bound serine protease from white croaker (Argyrosomus argentatus). Comp Biochem Physiol B Biochem Mol Biol. (2005) 141: 231-6.
Quantitative determination of the ligand content in Benzamidine Sepharose 4 Fast Flow media with ion-pair chromatography. J Chromatogr A. (2005) 1070: 103-9.
Partial purification and characterization of pro-phospholipase A2 activating proteases from gill membranes of the red sea bream, Chrysophrys major. Comp Biochem Physiol B Biochem Mol Biol. (2005) 141: 121-7.
Purification and characterization of jararassin-I, A thrombin-like enzyme from Bothrops jararaca snake venom. Acta Biochim Biophys Sin (Shanghai). (2004) 36: 798-802.
Preparation of recombinant alpha-thrombin: high-level expression of recombinant human prethrombin-2 and its activation by recombinant ecarin. J Biochem. (2004) 135: 577-82.
Purification and characterization of the fusion protein trypsin-streptavidin expressed in Escherichia coli. J Protein Chem. (2002) 21: 413-8.
Purification, characterization and crystallization of Jararacussin-I, a fibrinogen-clotting enzyme isolated from the venom of Bothrops jararacussu. Toxicon. (2002) 40: 1307-12.
Localization of a proteolytic enzyme within the efferent and deferent duct epithelial cells of the turkey (Meleagris gallopavo) using immunohistochemistry. Biol Reprod. (2002) 67: 276-81.
Xanthine oxidase and aldehyde oxidase: a simple procedure for the simultaneous purification from rat liver. Arch Biochem Biophys. (2002) 400: 48-53.
Construction and characterization of trifunctional single-chain urokinase-type plasminogen activators. Eur J Biochem. (2002) 269: 1708-13.
Construction and characterization of a recombinant chimeric plasminogen activator consisting of a fibrin peptide and a low molecular mass single-chain urokinase. Biochimie. (2001) 83: 1049-55.
Plasmin induces local thrombolysis without causing hemorrhage: a comparison with tissue plasminogen activator in the rabbit. Thromb Haemost. (2001) 86: 739-45.
Binding of plasminogen and tissue plasminogen activator to plasmin-modulated factor X and factor Xa. Biochemistry. (2001) 40: 6293-302.
Identification of a myofibril-bound serine proteinase (MBSP) in the skeletal muscle of lizard fish Saurida wanieso which specifically cleaves the arginine site. Comp Biochem Physiol B Biochem Mol Biol. (2000) 125: 255-64.
Purification and characterization of a trypsin-like serine proteinase from rat brain slices that degrades laminin and type IV collagen and stimulates protease-activated receptor-2. J Neurochem. (2000) 74: 1731-8.
Lactogenic hormones regulate xanthine oxidoreductase and beta-casein levels in mammary epithelial cells by distinct mechanisms. Arch Biochem Biophys. (2000) 373: 318-27.
Mouse mammary gland xanthine oxidoreductase: purification, characterization, and regulation. Arch Biochem Biophys. (1999) 371: 308-16.
Protease dependent activation of endothelial cells by peritoneal dialysis effluents. Thromb Haemost. (1999) 82: 1334-41.
Purification of mast cell proteases from murine skin. Exp Dermatol. (1999) 8: 413-8.
Spectrum of extracellular matrix degrading enzymes in normal and malignant T lymphocytes. Anticancer Res. (1999) 19: 1945-52.
Purification and further characterization of enteropeptidase from porcine duodenum. J Biochem. (1999) 125: 947-51.
Isolation and characterization of two digestive trypsin-like proteinases from larvae of the stalk corn borer, Sesamia nonagrioides. Insect Biochem Mol Biol. (1999) 29: 177-84.
Not more than three tissue kallikreins identified from organs of the guinea pig. Biol Chem. (1999) 380: 63-73.
Purification, characterization, and cloning of enamel matrix serine proteinase 1. J Dent Res. (1998) 77: 377-86.
Identification and activation of profollipsin, a latent precursor form of porcine follipsin. Eur J Biochem. (1997) 245: 392-7.
Purification and characterization of hepatic aldehyde oxidase in male and female mice. Arch Biochem Biophys. (1997) 338: 29-34.
Purification and characterization of hepsin from rat liver microsomes. Biochim Biophys Acta. (1997) 1337: 85-95.
Isolation and some molecular properties of a trypsin-like enzyme from larvae of European corn borer Ostrinia nubilalis Hübner (Lepidoptera: Pyralidae). Insect Biochem Mol Biol. (1996) 26: 883-9.
Purification of rat liver xanthine oxidase and xanthine dehydrogenase by affinity chromatography on benzamidine-sepharose. Arch Biochem Biophys. (1996) 332: 135-41.
Early trypsin, a female-specific midgut protease in Aedes aegypti: isolation, aminoterminal sequence determination, and cloning and sequencing of the gene. Insect Biochem Mol Biol. (1996) 26: 119-26.
Alpha 1-antitrypsin is degraded and non-functional in chronic wounds but intact and functional in acute wounds: the inhibitor protects fibronectin from degradation by chronic wound fluid enzymes. J Invest Dermatol. (1995) 105: 572-8.
Related products:
Benzamidine-Separopore® 6B (Columns) (SKU # 20170024)
Usage: bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product, food additive or as a household chemical
Hazmat Shipping : Non-hazardous
Storage : 2-8°C
Brand Name : bioPLUS™, Separopore®
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