beta actin Monoclonal Antibody

Background:
Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody can recognize all the actins. 

Research Area: Cancer, Signal Transduction, Tags and Cell Markers

Synonyms:
A26C1A, A26C1B, ACTB, ACTB, Actin beta, Actin cytoplasmic 1, Actin, cytoplasmic 1, N-terminally processed, Actx, b actin, Beta cytoskeletal actin, Beta-actin, BRWS1, E430023M04Rik, MGC128179, PS1TP5 binding protein 1, PS1TP5BP1

Immunogen: Synthetic Peptide

Swissprot: P60709

Calculated MW: 42 kDa
Observed MW: 43 kDa

Purification Method: Protein A purification

Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.

Concentration: 1 mg/mL
Dilution: WB 1:2000-1:5000, IHC 1:50-1:200

Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Citation(s):
http://www.ijcep.com/files/ijcep0044430.pdf
https://www.mdpi.com/1420-3049/24/1/121
https://www.spandidos-publications.com/ijmm/42/5/2737
https://pubmed.ncbi.nlm.nih.gov/29287795/
https://pubmed.ncbi.nlm.nih.gov/31720442/
https://pubmed.ncbi.nlm.nih.gov/31933822/
https://pubmed.ncbi.nlm.nih.gov/32579678/
https://pubmed.ncbi.nlm.nih.gov/32347916/
https://pubmed.ncbi.nlm.nih.gov/31943336/
https://pubmed.ncbi.nlm.nih.gov/32641048/