beta actin Polyclonal Antibody
€0.00
In stock
SKU
E-AB-20058
Catalog Number: E-AB-20058
Isotype: Rabbit IgG
Reactivity: human, mouse, rat, monkey, chicken, rabbit, sheep, xenopus
Applications: WB,IHC-p
Datasheet, Questions? Contact us!
Isotype: Rabbit IgG
Reactivity: human, mouse, rat, monkey, chicken, rabbit, sheep, xenopus
Applications: WB,IHC-p
Datasheet, Questions? Contact us!
Background:
Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody can recognize all the actins.
Research Area: Cancer, Signal Transduction, Tags and Cell Markers
Synonyms:
A26C1A, A26C1B, ACTB, ACTB, Actin beta, Actin cytoplasmic 1, Actin, cytoplasmic 1, N-terminally processed, Actx, b actin, Beta cytoskeletal actin, Beta-actin, BRWS1, E430023M04Rik, MGC128179, PS1TP5 binding protein 1, PS1TP5BP1
Immunogen: Recombinant Protein
Swissprot: P60709
Calculated MW: 42 kDa
Observed MW: 43 kDa
Purification Method: Affinity purification
Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Concentration: 1 mg/mL
Dilution: WB 1:500-1:2000, IHC 1:50-1:200
Storage:
Store at -20°C. Avoid freeze / thaw cycles.
Citation(s):
http://onlinelibrary.wiley.com/doi/10.1111/nep.12831/abstract
https://www.ncbi.nlm.nih.gov/pubmed/?term=Protective+effect+of+gentiopicroside+against+dextran+sodium+sulfate+induced+colitis+in+mice
https://molecular-cancer.biomedcentral.com/articles/10.1186/s12943-017-0639-2
https://www.sciencedirect.com/science/article/pii/S075333221733915X
https://www.mdpi.com/2218-273X/8/4/149
https://www.ncbi.nlm.nih.gov/pubmed/32003754
Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody can recognize all the actins.
Research Area: Cancer, Signal Transduction, Tags and Cell Markers
Synonyms:
A26C1A, A26C1B, ACTB, ACTB, Actin beta, Actin cytoplasmic 1, Actin, cytoplasmic 1, N-terminally processed, Actx, b actin, Beta cytoskeletal actin, Beta-actin, BRWS1, E430023M04Rik, MGC128179, PS1TP5 binding protein 1, PS1TP5BP1
Immunogen: Recombinant Protein
Swissprot: P60709
Calculated MW: 42 kDa
Observed MW: 43 kDa
Purification Method: Affinity purification
Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Concentration: 1 mg/mL
Dilution: WB 1:500-1:2000, IHC 1:50-1:200
Storage:
Store at -20°C. Avoid freeze / thaw cycles.
Citation(s):
http://onlinelibrary.wiley.com/doi/10.1111/nep.12831/abstract
https://www.ncbi.nlm.nih.gov/pubmed/?term=Protective+effect+of+gentiopicroside+against+dextran+sodium+sulfate+induced+colitis+in+mice
https://molecular-cancer.biomedcentral.com/articles/10.1186/s12943-017-0639-2
https://www.sciencedirect.com/science/article/pii/S075333221733915X
https://www.mdpi.com/2218-273X/8/4/149
https://www.ncbi.nlm.nih.gov/pubmed/32003754
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