Bim (phospho-S69/S65) polyclonal, anti-human, mouse, rat
€328.00
In stock
SKU
BS64039
Background:
Pro-apototic Bcl-2 family members promote cell death by neutralizing their anti-apoptotic relatives, which otherwise maintain cell viability by regulating caspase activity. Bim belongs to the BH3-only subgroup of Bcl-2 related proteins, and exists in three distinct isoforms, BimS (short), BimL (long) and BimEL (extra long). ERK1/2 phosphorylates BimEL, resulting in rapid degradation of the isoform via the proteasome pathway. At least three sites for ERK1/2 phosphorylation exist on BimEL, whereas ERK1/2 does not effect BimS and BimL, implying a unique role for BimEL in cell survival signaling.
Alternative Name:
Bcl-2-like protein 11, Bcl2-L-11, Bcl2-interacting mediator of cell death, BCL2L11, BIM
Application Dilution: WB: 1:500~1:1000
Specificity: p-Bim (S69/S65) polyclonal antibody detects endogenous levels of human Bim protein only when phosphorylated at Ser69. This antibody also recognizes mouse or rat Bim Protein when phosphorylated at Ser65.
Immunogen:
A synthetic peptide corresponding to residues in Human Bim around the phosphorylation site of Ser69.
MW: ~ 25 kDa
Swis Prot.: O43521
Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Format:
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.3.
Storage:
Store at 4°C short term. Aliquot and store at -22°C long term. Avoid freeze-thaw cycles.
For research use only, not for use in diagnostic procedure.
Pro-apototic Bcl-2 family members promote cell death by neutralizing their anti-apoptotic relatives, which otherwise maintain cell viability by regulating caspase activity. Bim belongs to the BH3-only subgroup of Bcl-2 related proteins, and exists in three distinct isoforms, BimS (short), BimL (long) and BimEL (extra long). ERK1/2 phosphorylates BimEL, resulting in rapid degradation of the isoform via the proteasome pathway. At least three sites for ERK1/2 phosphorylation exist on BimEL, whereas ERK1/2 does not effect BimS and BimL, implying a unique role for BimEL in cell survival signaling.
Alternative Name:
Bcl-2-like protein 11, Bcl2-L-11, Bcl2-interacting mediator of cell death, BCL2L11, BIM
Application Dilution: WB: 1:500~1:1000
Specificity: p-Bim (S69/S65) polyclonal antibody detects endogenous levels of human Bim protein only when phosphorylated at Ser69. This antibody also recognizes mouse or rat Bim Protein when phosphorylated at Ser65.
Immunogen:
A synthetic peptide corresponding to residues in Human Bim around the phosphorylation site of Ser69.
MW: ~ 25 kDa
Swis Prot.: O43521
Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Format:
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.3.
Storage:
Store at 4°C short term. Aliquot and store at -22°C long term. Avoid freeze-thaw cycles.
For research use only, not for use in diagnostic procedure.
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