Binding Buffer AnnexinV RUO
€0.00
In stock
SKU
IS-BB10X-50ML
PRODUCT DESCRIPTION
Exposure of PS on the external surface of the cell membrane has been reported in apoptotic cells, this occurs in the early phases of apoptotic cell dearth during which the cell membrane remains intact. In leukocyte apoptosis, PS on the outer surface of the cell marks the cell for recognition and phagocytosis by macrophages. Under defined salt and calcium conditions, annexin V can identify apoptotic cells by binding to PS exposed on the outer leaflet.
This product is used to facilitate the binding of annexin V to phosphatidylserine for use in apoptosis assays and is supplied as a 10X solution. It should be diluted to 1X in deionized water before using.
The pH of the 1X solution should fall within the range of pH 7.3-7.4. Adjust the pH if necessary. Warm the 1X solution to room temperature prior to use.
Samples must be prepared as single cell suspension in an appropriate tube.
Recommended usage:
Immunostep’s Annexin V binding buffer, is intended for flow cytometric labelling of apoptotic cells with Annexin V reagents.
Exposure of PS on the external surface of the cell membrane has been reported in apoptotic cells, this occurs in the early phases of apoptotic cell dearth during which the cell membrane remains intact. In leukocyte apoptosis, PS on the outer surface of the cell marks the cell for recognition and phagocytosis by macrophages. Under defined salt and calcium conditions, annexin V can identify apoptotic cells by binding to PS exposed on the outer leaflet.
This product is used to facilitate the binding of annexin V to phosphatidylserine for use in apoptosis assays and is supplied as a 10X solution. It should be diluted to 1X in deionized water before using.
The pH of the 1X solution should fall within the range of pH 7.3-7.4. Adjust the pH if necessary. Warm the 1X solution to room temperature prior to use.
Samples must be prepared as single cell suspension in an appropriate tube.
Recommended usage:
Immunostep’s Annexin V binding buffer, is intended for flow cytometric labelling of apoptotic cells with Annexin V reagents.
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