Biotinylated Human CD16a (C-His-Avi, 176V) recombinant protein
€554.00
In stock
SKU
BT-P1007H5
Background Information:
CD16a (FCGR3A) and its homolog CD16b are receptors for the Fc portion of human IgG. In contrast to CD64, which is a high affinity Fc binding protein, both CD16a and CD16b can bind Fc portion of human IgG with relative low affinity. Human CD16a is expressed mainly on natural killer cells, macrophages, T cells, and monocytes. While human CD16b is expressed on Neutrophils and eosinophils. In human cells, a single nucleotide polymorphism (T230A) creates a high binding (176V) and a low binding (176F) variant. Clinically, mutations in CD16a have been linked to vulnerability to viral infections, alloimmune neonatal neutropenia, and systemic lupus erythematosus.
Target Name: human CD16a, FCG3, FCGR3A, IGFR3
Source:
Human CD16a protein (accession number NP_P08637-1, natural variant Val176) (Gly17-Gln208) with C-terminus His tag and Avi tag is expressed in HEK293 cells. This protein was site specifically labeled with Biotin by BirA ligase.
Molecular Weight:
The 227 amino acid protein has the predicted molecular weight of 25.5 kDa. The protein migrates at approximately 40-50 kDa on SDS-PAGE with DTT-reduced condition.
Purity: >95% based on SDS-PAGE
Formulation: 1xPBS buffer, 0.22 µm filtered
Protein Concentration:
25µg size is bottled at 0.2mg/mL concentration. 100 µg size is bottled at lot specific concentration.
Storage and Handling:
Quick spin the vial after receiving. Unopened vial can be stored at 4°C for 2 weeks or at -20°C and below for six months. The protein can be further diluted to 0.1 mg/mL with 0.22 µm filtered Tris PH 7.5 buffer. This stock solution should be aliquoted and stored at ≤ -70°C to minimize the freeze/thaw cycles. Carrier protein such as FBS and BSA need to be added if further dilution is needed.
CD16a (FCGR3A) and its homolog CD16b are receptors for the Fc portion of human IgG. In contrast to CD64, which is a high affinity Fc binding protein, both CD16a and CD16b can bind Fc portion of human IgG with relative low affinity. Human CD16a is expressed mainly on natural killer cells, macrophages, T cells, and monocytes. While human CD16b is expressed on Neutrophils and eosinophils. In human cells, a single nucleotide polymorphism (T230A) creates a high binding (176V) and a low binding (176F) variant. Clinically, mutations in CD16a have been linked to vulnerability to viral infections, alloimmune neonatal neutropenia, and systemic lupus erythematosus.
Target Name: human CD16a, FCG3, FCGR3A, IGFR3
Source:
Human CD16a protein (accession number NP_P08637-1, natural variant Val176) (Gly17-Gln208) with C-terminus His tag and Avi tag is expressed in HEK293 cells. This protein was site specifically labeled with Biotin by BirA ligase.
Molecular Weight:
The 227 amino acid protein has the predicted molecular weight of 25.5 kDa. The protein migrates at approximately 40-50 kDa on SDS-PAGE with DTT-reduced condition.
Purity: >95% based on SDS-PAGE
Formulation: 1xPBS buffer, 0.22 µm filtered
Protein Concentration:
25µg size is bottled at 0.2mg/mL concentration. 100 µg size is bottled at lot specific concentration.
Storage and Handling:
Quick spin the vial after receiving. Unopened vial can be stored at 4°C for 2 weeks or at -20°C and below for six months. The protein can be further diluted to 0.1 mg/mL with 0.22 µm filtered Tris PH 7.5 buffer. This stock solution should be aliquoted and stored at ≤ -70°C to minimize the freeze/thaw cycles. Carrier protein such as FBS and BSA need to be added if further dilution is needed.
| Is Featured? | No |
|---|
Write Your Own Review