BL21 (DE3) Competent Cells

BL21 (DE3) Competent Cells are Chemically Competent Escherichia coli Cells used for Protein Expression of T7 RNA Polymerase-based Systems. These cells are resistant to the lytic bacteriophages T1 and T5. The BL21 (DE3) strain is a derivative of E. coli B. It is deficient in both lon and ompT proteases resulting in superior isolation of intact Recombinant Proteins. The host is a lysogen of DE3 and, therefore, carries a chromosomal copy of the T7 RNA polymerase gene that is controlled by the lacUV5 promoter. The strain utilizes the T7 RNA promoter to control protein expression. IPTG is used to induce Expression of the T7 RNA Polymerase.

Genotype: F¯ ompT gal dcm lon hsdSB(rB- mB-) λ(DE3 *lacI lacUV5-T7 gene 1 ind1 sam7 nin5])

Advantages & Features
- High-level of Protein Expression.
- Transformation Efficiency: ≥ 106 cfu/μg.
- Deficient in proteases Ion and OmpT.

Specifications
Genotype: F¯ ompT gal dcm lon hsdSB(rB- mB-) λ(DE3 *lacI lacUV5-T7 gene 1 ind1 sam7 nin5])

Includes
-10 µL BL21 (DE3) Competent Cells Control Plasmid (0.1 ng/µL)
- 10 x 200μL BL21 (DE3) Competent Cells
- Dry Ice

Applications
Protein Expression using the T7 Expression System.

Shipping & Storage
Shipment: with Dry Ice.
Storage: Upon receipt of the kit, immediately store the components at -80 °C. Storage at -20 °C will result in a significant decrease in transformation efficiency. Please note that Competent Cells are very sensitive to cycles of freezing and thawing and should not be exposed to temperature variations.
Extra charges for dry ice apply.