blaIMP + blaNDM-1 PCR Kit CE/IVD
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RealCycler IMND-
Catalog Number: RealCycler IMND-
1 Intended use:
RealCycler IMND is an in vitro diagnostic kit of reagents which allows real-time PCR qualitative detection of DNA from the blaIMP gene and blaNMD-1 gene simultaneously in clinical samples.
The system includes an internal control CHIC (Competitive Heterologous Internal Control) to prevent false negatives due to reaction inhibition.
2 Principle of the test
The polymerase chain reaction (PCR) is based on the amplification of a specific region of the DNA/RNA by using complementary primers to the target sequence. Real-time PCR uses marked probes with fluorophoresthat emit fluorescence in the case of amplification. The cycle of the PCR protocol in which appears significant fluorescence is proportional to the DNA/RNA quantity present in the sample. This value is called Cycle Threshold (Ct) or Cycle Quantification (Cq).
The amplification of blaIMP gene is detected in the corresponding channel of FAM fluorophore, blaNMD-1 gene is detected in the corresponding channel of TxR fluorophore and the internal control is detected in the corresponding channel of Alx532 or HEX fluorophore.
3 Technical specifications
Sensitivity
- blaIMP: 10 copies/μL
- blaNMD-1: 100 copies/μL.
Specificity:
- IMP: blaIMP genes (blaIMP-1, blaIMP-2, blaIMP-6, blaIMP-8, blaIMP-10, blaIMP-11, blaIMP-15, blaIMP-19, blaIMP-20, blaIMP-21, blaIMP-22, blaIMP-24, blaIMP-25, blaIMP-40, blaIMP-41 and blaIMP-42 it is not excluded the detection of other sequences of blaIMP group) and
- blaNDM-1: blaNDM-1 gene.
- Sensitivity determination method: Limit dilution,
- Reproducibility over 95% in repeated assays.
4 Contents
RealCycler IMND includes the AmpliMix and an IMND DNA Positive Control, which contains a mixture of blaIMP and blaNDM-1 DNA.
All reagents are ready to use without adding or rebuilding any component.
RealCycler IMND is an in vitro diagnostic kit of reagents which allows real-time PCR qualitative detection of DNA from the blaIMP gene and blaNMD-1 gene simultaneously in clinical samples.
The system includes an internal control CHIC (Competitive Heterologous Internal Control) to prevent false negatives due to reaction inhibition.
2 Principle of the test
The polymerase chain reaction (PCR) is based on the amplification of a specific region of the DNA/RNA by using complementary primers to the target sequence. Real-time PCR uses marked probes with fluorophoresthat emit fluorescence in the case of amplification. The cycle of the PCR protocol in which appears significant fluorescence is proportional to the DNA/RNA quantity present in the sample. This value is called Cycle Threshold (Ct) or Cycle Quantification (Cq).
The amplification of blaIMP gene is detected in the corresponding channel of FAM fluorophore, blaNMD-1 gene is detected in the corresponding channel of TxR fluorophore and the internal control is detected in the corresponding channel of Alx532 or HEX fluorophore.
3 Technical specifications
Sensitivity
- blaIMP: 10 copies/μL
- blaNMD-1: 100 copies/μL.
Specificity:
- IMP: blaIMP genes (blaIMP-1, blaIMP-2, blaIMP-6, blaIMP-8, blaIMP-10, blaIMP-11, blaIMP-15, blaIMP-19, blaIMP-20, blaIMP-21, blaIMP-22, blaIMP-24, blaIMP-25, blaIMP-40, blaIMP-41 and blaIMP-42 it is not excluded the detection of other sequences of blaIMP group) and
- blaNDM-1: blaNDM-1 gene.
- Sensitivity determination method: Limit dilution,
- Reproducibility over 95% in repeated assays.
4 Contents
RealCycler IMND includes the AmpliMix and an IMND DNA Positive Control, which contains a mixture of blaIMP and blaNDM-1 DNA.
All reagents are ready to use without adding or rebuilding any component.
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