Blood group A (clone 3-3A/33C13), anti-human

Source:
A BALB/c mouse was immunized with mucin isolated from an ovarian cyst fluid (pure endocervical type according to the Fenoglio’s classification).
Fusion partner: SP2/0.

Specifications:
3-3A reacts with determinants of chain A type 3 and 4 and chain H type 3 and 4, but not with type 1 and 2 chain structures. It is not reactive with immunodominant A trisaccharide. Increased expression of this antigen has been observed on some tumor tissues such as gastric carcinomas, urothelial carcinomas, and colon carcinomas. 3-3A does not react with normal tissue sections of donors with blood group B and 0 but it reacts specifically with malignant tissues in these individuals.

Species reactivity:
Positive: human.

Applications:
3-3A is applicable for tissue staining and immunofluorescence tests. 3-3A is not applicable for red cell agglutination.
Agglutination: -
Flow cytometry: +
Frozen sections: +
Immunofluorescence: +
Paraffin sections: Citrate

Positive control: KG1 cells or human colorectal carcinoma.

References:
- Blood transfusion and immunohematology, Ph Rouger, D Anstee and CH Salmon (Eds) – Arnette, France 30 (5) 252-720, (1987).
- Bara, J. et al. Biochem. J. 254:185-193 (1988).
- Yasumsds, I. et al. Glycoconjugate J. 3: 187-202 (2000).