Blood group A/Forssman (clone HE-193), anti-human

Source:
A BALB/c mouse was immunized with a mixture of erythrocytes of blood group A1 and a glycoprotein fraction isolated from the saliva of secretors with blood group A of human origin.
Fusion partner: P3-X63-Ag8.653.

Specifications:
HE-193 recognizes human blood group A (monofucosyl and difucosyl A antigens with chain types 1, 2, 3, 4, 5, 6,) and Forssmann antigen, which is normally not found in humans, but can appear on malignancies. De novo or increased expression of these antigens have been observed on some tumor tissues such as gastric carcinomas, urothelial carcinomas, and colon carcinomas. HE-193 does not react with normal tissue sections of donors with blood group B and 0 but it reacts specifically with malignant tissues in these individuals.

Species reactivity:
Positive: human.

Applications:
HE-193 is applicable for red cell agglutination, tissue staining and immunofluorescence tests.Agglutination
Flow cytometry: +
Frozen sections: +
Immunofluorescence: +
Paraffin sections: Citrate

Positive control: KG1 cells or human colorectal carcinoma.

References:
- Němec M. et al.Vox Sang52:125-8 (1987)