Blood group A type 3,4 + H type 3,4 (Clone HE-10), anti-human

Source:
A BALB/c mouse was immunized with a mixture of erythrocytes of blood group A1 and a glycoprotein fraction isolated from the saliva of secretors with blood group A of human origin.
Fusion partner: P3-X63-Ag8.653.

Specifications:
HE-10 preferably reacts with determinants of chain A type 3 and 4 and chain H type 3 and 4, but not with type 1 and 2 chain structures. It is not reactive with immunodominant A trisaccharide. Increased expression of this antigen has been observed on some tumor tissues such as gastric carcinomas, urothelial carcinomas, and colon carcinomas. HE-10 does not react with normal tissue sections of donors with blood group B and 0 but it reacts specifically with malignant tissues in these individuals.

Species reactivity:
Positive: human.

Applications:
HE-10 is applicable for red cell agglutination, tissue staining and immunofluorescence tests.Agglutination
Flow cytometry: +
Frozen sections: +
Immunofluorescence: +
Paraffin sections: Citrate

Positive control: KG1 cells or human colorectal carcinoma

References:
- Němec M. et al.Vox Sang52:125-8 (1987).
- Vanák J. et al.Neoplasma36(4): 479-487 (1989).
- Tichý, M. et al. Neoplasma37(4): 451-459 (1990).
- Tichý, M. et al. Acta Univ Palacki Olomuc Fac Med.126: 57-69 (1990)