Blue Separopore® 6B-CL

Application:
Cibacron Blue 3GA-Separopore® 6B-CL is an agarose conjugate in saline suspension used to searate and purify a variety of proteins. The ligand Cibacron Blue F3G-A is covalently coupled to Separopore® via chlorotriazine ring. The structure of the blue dye makes it a very versatile tool for separating many proteins, e.g. albumin, interferon, lipoproteins and blood coagulation factors. Blue-Separopore® 6B-CL is used for purification of albumin, enzymes (including NAD+ and NADP+), coagulation factors, interferons and related proteins. Due to a broad spectrum of the affinity, this product can be used as a general purpose protein purification tool. The cross-linked matrix provides a stable, rigid medium even in the presence of dissociating agents.

Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics

Technical Specifications:
Ligand: Cibacron Blue F3G-A
Matrix: Separopore® 6B-CL (crosslinked agarose, 6%)
Particle size range: 53 – 180 µm
Matrix activation: Triazine coupling
Coupling conditions: pH 9 – 10
Ligand density: 8 µmol Cibacron Blue/ml drained gel
Binding capacity: > 15 – 20 mg bovine serum albumin/ml drained gel
pH stability: 3 – 13
Chemical stability: Stable to all commonly used aqueous buffers and additives such as 8M urea and 6M guanidine hydrochloride.
Physical stability: Negligible volume variation due to changes in pH or ionic strength.
Supplied as Suspension in 20% ethanol
Storage: 2 – 8 °C

References:
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Purification and characterization of an endo-D:-arabinase produced by cellulomonas. Protein J. (2012) 31: 51-8.
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Purification and characterization of hemolymph prophenoloxidase from Ostrinia furnacalis (Lepidoptera: Pyralidae) larvae. Comp Biochem Physiol B Biochem Mol Biol. (2008) 151: 139-46.
Characterization and large-scale production of recombinant Streptoverticillium platensis transglutaminase. J Ind Microbiol Biotechnol. (2008) 35: 981-90.
Purification and characterization of glyceraldehyde-3-phosphate dehydrogenase from European pilchard Sardina pilchardus. Acta Biochim Biophys Sin (Shanghai). (2007) 39: 947-54.
Purification and biochemical characterization of ovine alpha-1-proteinase inhibitor: mechanistic adaptations and role of Phe350 and Met356. Protein Expr Purif. (2008) 57: 290-302.
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A liver-derived immunosuppressive factor is an arginase: identification and mechanism of immunosuppression. Biomed Res. (2007) 28: 17-23.
Purification and characterization of cytosolic glyceraldehyde-3-phosphate dehydrogenase from the dromedary camel. Acta Biochim Biophys Sin (Shanghai). (2007) 39: 148-54.
Induced binding of proteins by ammonium sulfate in affinity and ion-exchange column chromatography. J Biochem Biophys Methods. (2007) 70: 493-8.
Improved performance of column chromatography by arginine: dye-affinity chromatography. Protein Expr Purif. (2007) 52: 410-4.
Preparation of convection interaction media isobutyl disc monolithic column and its application to purification of secondary alcohol dehydrogenase and alcohol oxidase. J Chromatogr A. (2007) 1144: 85-9.
Temperature dependence of nitrate reductase in the psychrophilic unicellular alga Koliella antarctica and the mesophilic alga Chlorella sorokiniana. Plant Cell Environ. (2006) 29: 1400-9.
Heterogeneity of Escherichia coli-expressed human muscle creatine kinase. IUBMB Life. (2006) 58: 421-8.
Monosize poly(glycidyl methacrylate) beads for dye-affinity purification of lysozyme. Int J Biol Macromol. (2006) 38: 99-106.
Formation of albumin dimers induced by exposure to peroxides in human plasma: a possible biomarker for oxidative stress. Biochem Biophys Res Commun. (2006) 340: 353-8.
Effect of hexanol on the reversed micelles of Span 85 modified with Cibacron Blue F-3GA for protein solubilization. J Colloid Interface Sci. (2006) 297: 805-12.
Purification and some properties of sulfur reductase from the iron-oxidizing bacterium Thiobacillus ferrooxidans NASF-1. J Biosci Bioeng. (2000) 90: 199-203.
The structure and function of oxidized albumin in hemodialysis patients: Its role in elevated oxidative stress via neutrophil burst. Biochem Biophys Res Commun. (2005) 334: 1322-8.
Process intensification of fluidized bed dye-ligand adsorption of G3PDH from unclarified disrupted yeast: a case study of the performance of a high-density steel-agarose pellicular adsorbent. Protein Expr Purif. (2005) 42: 160-5.
Identification of oligopeptidase B in higher plants. Purification and characterization of oligopeptidase B from quiescent wheat embryo, Triticum aestivum. J Biochem. (2004) 136: 673-81.
Establishment of a large-scale purification procedure for purified recombinant bovine interferon-tau produced by a silkworm-baculovirus gene expression system. J Vet Med Sci. (2004) 66: 1395-401.
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Oral streptococcal glyceraldehyde-3-phosphate dehydrogenase mediates interaction with Porphyromonas gingivalis fimbriae. Microbes Infect. (2004) 6: 1163-70.
Tissue distribution and purification of prophenoloxidase in larvae of Asian Corn Borer, Ostrinia furnacalis Guenee (Lepidoptera: Pyralidae). Acta Biochim Biophys Sin (Shanghai). (2004) 36: 360-4.
The recombinant subdomain IIIB of human serum albumin displays activity of gonadotrophin surge-attenuating factor. Hum Reprod. (2004) 19: 849-58.
Expression of ricin A chain and ricin A chain-KDEL in Escherichia coli. Protein Expr Purif. (2004) 34: 197-201.
A model for the salt effect on adsorption equilibrium of basic protein to dye-ligand affinity adsorbent. Biotechnol Prog. (2004) 20: 207-14.
A trans-Golgi network retention signal YQRL fused to ricin A chain significantly enhances its cytotoxicity. Biochem Biophys Res Commun. (2004) 313: 1053-7.

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Usage: bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.

Hazmat Shipping : Non-hazardous
Storage : 2-8°C
Appearance Form : Liquid
Appearance Color : Blue
Brand Name : bioPLUS™, Separopore®