Blue Separopore® 6B-CL OnePass™ Column
€0.00
In stock
SKU
BW-20181147
Application:
Cibacron Blue 3GA-Separopore® 6B-CL is an agarose conjugate in saline suspension used to searate and purify a variety of proteins. The ligand Cibacron Blue F3G-A is covalently coupled to Separopore® via chlorotriazine ring. The structure of the blue dye makes it a very versatile tool for separating many proteins, e.g. albumin, interferon, lipoproteins and blood coagulation factors. Blue-Separopore® 6B-CL is used for purification of albumin, enzymes (including NAD+ and NADP+), coagulation factors, interferons and related proteins. Due to a broad spectrum of the affinity, this product can be used as a general purpose protein purification tool. The cross-linked matrix provides a stable, rigid medium even in the presence of dissociating agents.
Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.
Technical Specifications:
Ligand: Cibacron Blue F3G-A
Matrix: Separopore® 6B-CL (crosslinked agarose, 6%)
Particle size range: 52 – 165 µm
Matrix activation: Triazine coupling
Coupling conditions: pH 9 – 10
Ligand density: 8 µmol Cibacron Blue/ml drained gel
Binding capacity: > 15 – 20 mg bovine serum albumin/ml drained gel
pH stability: 3 – 13
Chemical stability: Stable to all commonly used aqueous buffers and additives such as 8M urea and 6M guanidine hydrochloride.
Physical stability: Negligible volume variation due to changes in pH or ionic strength.
Supplied as Suspension in 0.5M NaCl containing 0.02% thimerosal
Storage: 2 – 8 °C
References:
Elution relationships to model affinity chromatography using a general rate model. J Mol Recognit. (2012) 25: 571-9.
Expression and purification of recombinant human serum albumin fusion protein with VEGF165b in Pichia pastoris. Protein Expr Purif. (2012) 85: 32-7.
Stereoselective binding of mexiletine and ketoprofen enantiomers with human serum albumin domains. Acta Pharmacol Sin. (2012) 33: 710-6.
Purification and characterization of an endo-D:-arabinase produced by cellulomonas. Protein J. (2012) 31: 51-8.
Cibacron Blue and proteomics: the mystery of the platoon missing in action. J Proteomics. (2011) 74: 2856-65.
Determination of robustness and optimal work conditions for a purification process of a therapeutic recombinant protein using response surface methodology. Biotechnol Prog. (2011) 27: 724-32.
An alternative method for purifying and detoxifying diphtheria toxin. Toxicon. (2011) 57: 1093-100.
Simple method for isolation of glyceraldehyde 3-phosphate dehydrogenase and the improvement of myofibril gel properties. Anim Sci J. (2011) 82: 136-43.
Extension of the selection of protein chromatography and the rate model to affinity chromatography. J Mol Recognit. (2010) 23: 609-17.
Inhibition of neutrophil migration by hemopexin leads to increased mortality due to sepsis in mice. Am J Respir Crit Care Med. (2011) 183: 922-31.
Identification, cloning and characterization of an aldo-keto reductase from Trypanosoma cruzi with quinone oxido-reductase activity. Mol Biochem Parasitol. (2010) 173: 132-41.
Bioactivity and pharmacokinetics of two human serum albumin-thymosin alpha1-fusion proteins, rHSA-Talpha1 and rHSA-L-Talpha1, expressed in recombinant Pichia pastoris. Cancer Immunol Immunother. (2010) 59: 1335-45.
Structural and catalytic properties of the D-3-hydroxybutyrate dehydrogenase from Pseudomonas aeruginosa. Curr Microbiol. (2010) 61: 7-12.
Analysis of nucleoside-binding proteins by ligand-specific elution from dye resin: application to Mycobacterium tuberculosis aldehyde dehydrogenases. J Struct Funct Genomics. (2009) 10: 291-301.
Isolation and characterization of a French bean hemagglutinin with antitumor, antifungal, and anti-HIV-1 reverse transcriptase activities and an exceptionally high yield. Phytomedicine. (2010) 17: 457-62.
Proteomics-based approach for identification and purification of human phosphate binding apolipoprotein from amniotic fluid. Genet Mol Res. (2009) 8: 929-37.
Immunoaffinity purification and characterization of glyceraldehyde-3-phosphate dehydrogenase from human erythrocytes. Acta Biochim Biophys Sin(Shanghai). (2009) 41: 399-406.
Purification and characterization of hemolymph prophenoloxidase from Ostrinia furnacalis (Lepidoptera: Pyralidae) larvae. Comp Biochem Physiol B Biochem Mol Biol. (2008) 151: 139-46.
Characterization and large-scale production of recombinant Streptoverticillium platensis transglutaminase. J Ind Microbiol Biotechnol. (2008) 35: 981-90.
Purification and characterization of glyceraldehyde-3-phosphate dehydrogenase from European pilchard Sardina pilchardus. Acta Biochim Biophys Sin(Shanghai). (2007) 39: 947-54.
Purification and biochemical characterization of ovine alpha-1-proteinase inhibitor: mechanistic adaptations and role of Phe350 and Met356. Protein Expr Purif. (2008) 57: 290-302.
Nature of non-transferrin-bound iron: studies on iron citrate complexes and thalassemic sera. J Biol Inorg Chem. (2008) 13: 57-74.
L-2-hydroxyglutaric aciduria, a defect of metabolite repair. J Inherit Metab Dis. (2007) 30: 681-9.
A novel NADH-dependent carbonyl reductase with unusual stereoselectivity for (R)-specific reduction from an (S)-1-phenyl-1,2-ethanediol-producing micro-organism: purification and characterization. Lett Appl Microbiol. (2007) 44: 555-62.
A liver-derived immunosuppressive factor is an arginase: identification and mechanism of immunosuppression. Biomed Res. (2007) 28: 17-23.
Purification and characterization of cytosolic glyceraldehyde-3-phosphate dehydrogenase from the dromedary camel. Acta Biochim Biophys Sin (Shanghai). (2007) 39: 148-54.
Induced binding of proteins by ammonium sulfate in affinity and ion-exchange column chromatography. J Biochem Biophys Methods. (2007) 70: 493-8.
Improved performance of column chromatography by arginine: dye-affinity chromatography. Protein Expr Purif. (2007) 52: 410-4.
Preparation of convection interaction media isobutyl disc monolithic column and its application to purification of secondary alcohol dehydrogenase and alcohol oxidase. J Chromatogr A. (2007) 1144: 85-9.
Temperature dependence of nitrate reductase in the psychrophilic unicellular alga Koliella antarctica and the mesophilic alga Chlorella sorokiniana. Plant Cell Environ. (2006) 29: 1400-9.
Heterogeneity of Escherichia coli-expressed human muscle creatine kinase. IUBMB Life. (2006) 58: 421-8.
Monosize poly(glycidyl methacrylate) beads for dye-affinity purification of lysozyme. Int J Biol Macromol. (2006) 38: 99-106.
Formation of albumin dimers induced by exposure to peroxides in human plasma: a possible biomarker for oxidative stress. Biochem Biophys Res Commun. (2006) 340: 353-8.
Effect of hexanol on the reversed micelles of Span 85 modified with Cibacron Blue F-3GA for protein solubilization. J Colloid Interface Sci. (2006) 297: 805-12.
Purification and some properties of sulfur reductase from the iron-oxidizing bacterium Thiobacillus ferrooxidans NASF-1. J Biosci Bioeng. (2000) 90: 199-203.
The structure and function of oxidized albumin in hemodialysis patients: Its role in elevated oxidative stress via neutrophil burst. Biochem Biophys Res Commun. (2005) 334: 1322-8.
Process intensification of fluidized bed dye-ligand adsorption of G3PDH from unclarified disrupted yeast: a case study of the performance of a high-density steel-agarose pellicular adsorbent. Protein Expr Purif. (2005) 42: 160-5.
Identification of oligopeptidase B in higher plants. Purification and characterization of oligopeptidase B from quiescent wheat embryo, Triticum aestivum. J Biochem. (2004) 136: 673-81.
Establishment of a large-scale purification procedure for purified recombinant bovine interferon-tau produced by a silkworm-baculovirus gene expression system. J Vet Med Sci. (2004) 66: 1395-401.
Lactoferrin is the major deoxyribonuclease of human milk. Biochemistry (Mosc). (2004) 69: 1006-15.
Oral streptococcal glyceraldehyde-3-phosphate dehydrogenase mediates interaction with Porphyromonas gingivalis fimbriae. Microbes Infect. (2004) 6: 1163-70.
Tissue distribution and purification of prophenoloxidase in larvae of Asian Corn Borer, Ostrinia furnacalis Guenee (Lepidoptera: Pyralidae). Acta Biochim Biophys Sin (Shanghai). (2004) 36: 360-4.
The recombinant subdomain IIIB of human serum albumin displays activity of gonadotrophin surge-attenuating factor. Hum Reprod. (2004) 19: 849-58.
Expression of ricin A chain and ricin A chain-KDEL in Escherichia coli. Protein Expr Purif. (2004) 34: 197-201.
A model for the salt effect on adsorption equilibrium of basic protein to dye-ligand affinity adsorbent. Biotechnol Prog. (2004) 20: 207-14.
A trans-Golgi network retention signal YQRL fused to ricin A chain significantly enhances its cytotoxicity. Biochem Biophys Res Commun. (2004) 313: 1053-7.
Hazmat Shipping : Non-hazardous
Storage : 2-8°C
Brand Name : bioPLUS™, Separopore®
Cibacron Blue 3GA-Separopore® 6B-CL is an agarose conjugate in saline suspension used to searate and purify a variety of proteins. The ligand Cibacron Blue F3G-A is covalently coupled to Separopore® via chlorotriazine ring. The structure of the blue dye makes it a very versatile tool for separating many proteins, e.g. albumin, interferon, lipoproteins and blood coagulation factors. Blue-Separopore® 6B-CL is used for purification of albumin, enzymes (including NAD+ and NADP+), coagulation factors, interferons and related proteins. Due to a broad spectrum of the affinity, this product can be used as a general purpose protein purification tool. The cross-linked matrix provides a stable, rigid medium even in the presence of dissociating agents.
Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.
Technical Specifications:
Ligand: Cibacron Blue F3G-A
Matrix: Separopore® 6B-CL (crosslinked agarose, 6%)
Particle size range: 52 – 165 µm
Matrix activation: Triazine coupling
Coupling conditions: pH 9 – 10
Ligand density: 8 µmol Cibacron Blue/ml drained gel
Binding capacity: > 15 – 20 mg bovine serum albumin/ml drained gel
pH stability: 3 – 13
Chemical stability: Stable to all commonly used aqueous buffers and additives such as 8M urea and 6M guanidine hydrochloride.
Physical stability: Negligible volume variation due to changes in pH or ionic strength.
Supplied as Suspension in 0.5M NaCl containing 0.02% thimerosal
Storage: 2 – 8 °C
References:
Elution relationships to model affinity chromatography using a general rate model. J Mol Recognit. (2012) 25: 571-9.
Expression and purification of recombinant human serum albumin fusion protein with VEGF165b in Pichia pastoris. Protein Expr Purif. (2012) 85: 32-7.
Stereoselective binding of mexiletine and ketoprofen enantiomers with human serum albumin domains. Acta Pharmacol Sin. (2012) 33: 710-6.
Purification and characterization of an endo-D:-arabinase produced by cellulomonas. Protein J. (2012) 31: 51-8.
Cibacron Blue and proteomics: the mystery of the platoon missing in action. J Proteomics. (2011) 74: 2856-65.
Determination of robustness and optimal work conditions for a purification process of a therapeutic recombinant protein using response surface methodology. Biotechnol Prog. (2011) 27: 724-32.
An alternative method for purifying and detoxifying diphtheria toxin. Toxicon. (2011) 57: 1093-100.
Simple method for isolation of glyceraldehyde 3-phosphate dehydrogenase and the improvement of myofibril gel properties. Anim Sci J. (2011) 82: 136-43.
Extension of the selection of protein chromatography and the rate model to affinity chromatography. J Mol Recognit. (2010) 23: 609-17.
Inhibition of neutrophil migration by hemopexin leads to increased mortality due to sepsis in mice. Am J Respir Crit Care Med. (2011) 183: 922-31.
Identification, cloning and characterization of an aldo-keto reductase from Trypanosoma cruzi with quinone oxido-reductase activity. Mol Biochem Parasitol. (2010) 173: 132-41.
Bioactivity and pharmacokinetics of two human serum albumin-thymosin alpha1-fusion proteins, rHSA-Talpha1 and rHSA-L-Talpha1, expressed in recombinant Pichia pastoris. Cancer Immunol Immunother. (2010) 59: 1335-45.
Structural and catalytic properties of the D-3-hydroxybutyrate dehydrogenase from Pseudomonas aeruginosa. Curr Microbiol. (2010) 61: 7-12.
Analysis of nucleoside-binding proteins by ligand-specific elution from dye resin: application to Mycobacterium tuberculosis aldehyde dehydrogenases. J Struct Funct Genomics. (2009) 10: 291-301.
Isolation and characterization of a French bean hemagglutinin with antitumor, antifungal, and anti-HIV-1 reverse transcriptase activities and an exceptionally high yield. Phytomedicine. (2010) 17: 457-62.
Proteomics-based approach for identification and purification of human phosphate binding apolipoprotein from amniotic fluid. Genet Mol Res. (2009) 8: 929-37.
Immunoaffinity purification and characterization of glyceraldehyde-3-phosphate dehydrogenase from human erythrocytes. Acta Biochim Biophys Sin(Shanghai). (2009) 41: 399-406.
Purification and characterization of hemolymph prophenoloxidase from Ostrinia furnacalis (Lepidoptera: Pyralidae) larvae. Comp Biochem Physiol B Biochem Mol Biol. (2008) 151: 139-46.
Characterization and large-scale production of recombinant Streptoverticillium platensis transglutaminase. J Ind Microbiol Biotechnol. (2008) 35: 981-90.
Purification and characterization of glyceraldehyde-3-phosphate dehydrogenase from European pilchard Sardina pilchardus. Acta Biochim Biophys Sin(Shanghai). (2007) 39: 947-54.
Purification and biochemical characterization of ovine alpha-1-proteinase inhibitor: mechanistic adaptations and role of Phe350 and Met356. Protein Expr Purif. (2008) 57: 290-302.
Nature of non-transferrin-bound iron: studies on iron citrate complexes and thalassemic sera. J Biol Inorg Chem. (2008) 13: 57-74.
L-2-hydroxyglutaric aciduria, a defect of metabolite repair. J Inherit Metab Dis. (2007) 30: 681-9.
A novel NADH-dependent carbonyl reductase with unusual stereoselectivity for (R)-specific reduction from an (S)-1-phenyl-1,2-ethanediol-producing micro-organism: purification and characterization. Lett Appl Microbiol. (2007) 44: 555-62.
A liver-derived immunosuppressive factor is an arginase: identification and mechanism of immunosuppression. Biomed Res. (2007) 28: 17-23.
Purification and characterization of cytosolic glyceraldehyde-3-phosphate dehydrogenase from the dromedary camel. Acta Biochim Biophys Sin (Shanghai). (2007) 39: 148-54.
Induced binding of proteins by ammonium sulfate in affinity and ion-exchange column chromatography. J Biochem Biophys Methods. (2007) 70: 493-8.
Improved performance of column chromatography by arginine: dye-affinity chromatography. Protein Expr Purif. (2007) 52: 410-4.
Preparation of convection interaction media isobutyl disc monolithic column and its application to purification of secondary alcohol dehydrogenase and alcohol oxidase. J Chromatogr A. (2007) 1144: 85-9.
Temperature dependence of nitrate reductase in the psychrophilic unicellular alga Koliella antarctica and the mesophilic alga Chlorella sorokiniana. Plant Cell Environ. (2006) 29: 1400-9.
Heterogeneity of Escherichia coli-expressed human muscle creatine kinase. IUBMB Life. (2006) 58: 421-8.
Monosize poly(glycidyl methacrylate) beads for dye-affinity purification of lysozyme. Int J Biol Macromol. (2006) 38: 99-106.
Formation of albumin dimers induced by exposure to peroxides in human plasma: a possible biomarker for oxidative stress. Biochem Biophys Res Commun. (2006) 340: 353-8.
Effect of hexanol on the reversed micelles of Span 85 modified with Cibacron Blue F-3GA for protein solubilization. J Colloid Interface Sci. (2006) 297: 805-12.
Purification and some properties of sulfur reductase from the iron-oxidizing bacterium Thiobacillus ferrooxidans NASF-1. J Biosci Bioeng. (2000) 90: 199-203.
The structure and function of oxidized albumin in hemodialysis patients: Its role in elevated oxidative stress via neutrophil burst. Biochem Biophys Res Commun. (2005) 334: 1322-8.
Process intensification of fluidized bed dye-ligand adsorption of G3PDH from unclarified disrupted yeast: a case study of the performance of a high-density steel-agarose pellicular adsorbent. Protein Expr Purif. (2005) 42: 160-5.
Identification of oligopeptidase B in higher plants. Purification and characterization of oligopeptidase B from quiescent wheat embryo, Triticum aestivum. J Biochem. (2004) 136: 673-81.
Establishment of a large-scale purification procedure for purified recombinant bovine interferon-tau produced by a silkworm-baculovirus gene expression system. J Vet Med Sci. (2004) 66: 1395-401.
Lactoferrin is the major deoxyribonuclease of human milk. Biochemistry (Mosc). (2004) 69: 1006-15.
Oral streptococcal glyceraldehyde-3-phosphate dehydrogenase mediates interaction with Porphyromonas gingivalis fimbriae. Microbes Infect. (2004) 6: 1163-70.
Tissue distribution and purification of prophenoloxidase in larvae of Asian Corn Borer, Ostrinia furnacalis Guenee (Lepidoptera: Pyralidae). Acta Biochim Biophys Sin (Shanghai). (2004) 36: 360-4.
The recombinant subdomain IIIB of human serum albumin displays activity of gonadotrophin surge-attenuating factor. Hum Reprod. (2004) 19: 849-58.
Expression of ricin A chain and ricin A chain-KDEL in Escherichia coli. Protein Expr Purif. (2004) 34: 197-201.
A model for the salt effect on adsorption equilibrium of basic protein to dye-ligand affinity adsorbent. Biotechnol Prog. (2004) 20: 207-14.
A trans-Golgi network retention signal YQRL fused to ricin A chain significantly enhances its cytotoxicity. Biochem Biophys Res Commun. (2004) 313: 1053-7.
Hazmat Shipping : Non-hazardous
Storage : 2-8°C
Brand Name : bioPLUS™, Separopore®
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