Bordetella pertussis + Bordetella parapertussis PCR Kit CE/IVD
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In stock
SKU
RealCycler BORD-
Catalog Number: RealCycler BORD-
1 Intended use:
RealCycler BORD is an in vitro diagnostic kit of reagents which allows real-time PCR detection of Bordetella pertussis and Bordetella parapertussis DNA simultaneously in clinical samples.
Bordetella pertussis and Bordetella parapertussis pathogens could be quantified if this product is used in combination with the corresponding calibrator (references RealCycler BPEQ or BPAQ respectively).
The system includes an internal control CHIC (Competitive Heterologous Internal Control) to prevent false negatives due to reaction inhibition.
2 Principle of the test
The polymerase chain reaction (PCR) is based on the amplification of a specific region of the DNA/RNA by using complementary primers to the target sequence. Real-time PCR uses marked probes with fluorophores that emit fluorescence in the case of amplification. The cycle of the PCR protocol in which appears significant fluorescence is proportional to the DNA/RNA quantity present in the sample. This value is called Cycle Threshold (Ct) or Cycle Quantification (Cq).
The amplification of Bordetella pertussis is detected in the corresponding channel of FAM fluorophore, CHIC is detected in the corresponding channel of Alx532 fluorophore and Bordetella parapertussis is detected in the corresponding channel of TxR fluorophore.
3 Technical specifications
Sensitivity:
- Bordetella pertussis: 10 copies/μL.
- Bordetella parapertussis: 1 copy/μL.
The analytical sensitivity has been determined by limit dilution. This sensitivity has been showed in repeated assays with reproducibility over 95%.
Specificity:
- Bordetella pertussis: BP283 region.
- Bordetella parapertussis: IS1001.
Specificity validation has been performed according to experimental assays and BLAST analysis (www.ncbi.nlm.nih.gov/blast).
4. Contents
RealCycler BORD includes the BORD AmpliMix dispensed into amplification tubes and a BORD DNA Positive Control, which contains a mixture of Bordetella pertussis and Bordetella parapertussisDNA.
All reagents are ready to use without adding or rebuilding any component.
Sample material: Purified DNA from clinical samples.
RealCycler BORD is an in vitro diagnostic kit of reagents which allows real-time PCR detection of Bordetella pertussis and Bordetella parapertussis DNA simultaneously in clinical samples.
Bordetella pertussis and Bordetella parapertussis pathogens could be quantified if this product is used in combination with the corresponding calibrator (references RealCycler BPEQ or BPAQ respectively).
The system includes an internal control CHIC (Competitive Heterologous Internal Control) to prevent false negatives due to reaction inhibition.
2 Principle of the test
The polymerase chain reaction (PCR) is based on the amplification of a specific region of the DNA/RNA by using complementary primers to the target sequence. Real-time PCR uses marked probes with fluorophores that emit fluorescence in the case of amplification. The cycle of the PCR protocol in which appears significant fluorescence is proportional to the DNA/RNA quantity present in the sample. This value is called Cycle Threshold (Ct) or Cycle Quantification (Cq).
The amplification of Bordetella pertussis is detected in the corresponding channel of FAM fluorophore, CHIC is detected in the corresponding channel of Alx532 fluorophore and Bordetella parapertussis is detected in the corresponding channel of TxR fluorophore.
3 Technical specifications
Sensitivity:
- Bordetella pertussis: 10 copies/μL.
- Bordetella parapertussis: 1 copy/μL.
The analytical sensitivity has been determined by limit dilution. This sensitivity has been showed in repeated assays with reproducibility over 95%.
Specificity:
- Bordetella pertussis: BP283 region.
- Bordetella parapertussis: IS1001.
Specificity validation has been performed according to experimental assays and BLAST analysis (www.ncbi.nlm.nih.gov/blast).
4. Contents
RealCycler BORD includes the BORD AmpliMix dispensed into amplification tubes and a BORD DNA Positive Control, which contains a mixture of Bordetella pertussis and Bordetella parapertussisDNA.
All reagents are ready to use without adding or rebuilding any component.
Sample material: Purified DNA from clinical samples.
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