BRAF (clone 1H12), anti-human, mouse
€411.00
In stock
SKU
252864
Catalog Nr.: 252864
Size: 0.1 ml
Isotype: Mouse IgG1
Applications: E, WB, IHC, IF
Size: 0.1 ml
Isotype: Mouse IgG1
Applications: E, WB, IHC, IF
Protein Family: Enzymes, Protein Kinases
Pathway and Disease: Signal Transduction, Cell Communication, Cell Motility, Nervous and Sensory Systems, Endocrine System, Immune System, Immune System
Description:
BRAF (v-raf murine sarcoma viral oncogene homolog B1) is the main effectors recruited by GTP-bound Ras to activate the MEK-MAP kinase pathway. B-Raf contains three consensus Akt phosphorylationsites (Ser364, Ser428 and Thr439). B-Raf is a key regulatory molecule of the mitogen-activated protein kinase kinase (MEK), it has a long amino-terminal region, the region is essential for homo-dimerization of B-Raf and hetero-dimerization of B-Raf and c-Raf at the plasma membrane, followed by phosphorylation of Thr118 in the amino-terminal B-Raf-specific region. Notably, in calcium ionophore-stimulated HeLa cells, B-Raf could propagate signals to MEK under the basal level of GTP-Ras. Expression of Raf-B is highly restricted with highest levels in the cerebrum and testes and defects in braf are involved in a wide range of cancers. The BRAF gene mutation is frequently detected in papillary thyroid carcinoma, melanocytic nevi, primary cutaneous melanomas and colorectal cancers.
Alternate Names: RAF proto-oncogene serine/threonine-protein kinase, Proto-oncogene c-RAF, cRaf, Raf-1, Raf
Application Notes: E: 1:10000; WB: 1:500-1:2000; IHC: 1:200-1:1000; IF: 1:200-1:1000
Accession No.: P11345
Antigen: Purified recombinant fragment of human BRAF expressed in E. coli.
Format: Each vial contains 0.1 ml ascitic fluid with 0.03% sodium azide.
Storage:
Store at 4°C for short term use only. Store at -20°C for storage over 1 month. Product is guaranteed 6 months from the date of shipment.
Pathway and Disease: Signal Transduction, Cell Communication, Cell Motility, Nervous and Sensory Systems, Endocrine System, Immune System, Immune System
Description:
BRAF (v-raf murine sarcoma viral oncogene homolog B1) is the main effectors recruited by GTP-bound Ras to activate the MEK-MAP kinase pathway. B-Raf contains three consensus Akt phosphorylationsites (Ser364, Ser428 and Thr439). B-Raf is a key regulatory molecule of the mitogen-activated protein kinase kinase (MEK), it has a long amino-terminal region, the region is essential for homo-dimerization of B-Raf and hetero-dimerization of B-Raf and c-Raf at the plasma membrane, followed by phosphorylation of Thr118 in the amino-terminal B-Raf-specific region. Notably, in calcium ionophore-stimulated HeLa cells, B-Raf could propagate signals to MEK under the basal level of GTP-Ras. Expression of Raf-B is highly restricted with highest levels in the cerebrum and testes and defects in braf are involved in a wide range of cancers. The BRAF gene mutation is frequently detected in papillary thyroid carcinoma, melanocytic nevi, primary cutaneous melanomas and colorectal cancers.
Alternate Names: RAF proto-oncogene serine/threonine-protein kinase, Proto-oncogene c-RAF, cRaf, Raf-1, Raf
Application Notes: E: 1:10000; WB: 1:500-1:2000; IHC: 1:200-1:1000; IF: 1:200-1:1000
Accession No.: P11345
Antigen: Purified recombinant fragment of human BRAF expressed in E. coli.
Format: Each vial contains 0.1 ml ascitic fluid with 0.03% sodium azide.
Storage:
Store at 4°C for short term use only. Store at -20°C for storage over 1 month. Product is guaranteed 6 months from the date of shipment.
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