Brown Separopore® 4B-CL
€0.00
In stock
SKU
BW-20181100
Application:
Brown-Separopore® 4B-CL is a dye-ligand affinity matrix consisting of Reactive Brown 10 bound to cross-linked 4% agarose beads. It is used to purify proteins that have distinctive affinity to this dye. Immobilized dyes have been found to bind from 5 to 60 % of the proteins in various crude cell extracts. The affinity for reactive dyes to proteins may be due to substrate /cofactor similarities as well as hydrophobic and ion exchange properties. Some proteins have been found to require the addition of divalent cations for binding to dye resins.
Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.
Technical Specifications:
Ligand: Reactive Brown 10
Matrix: Separopore® 4B-CL (crosslinked agarose beads, 4%)
Particle size range: 52 – 165 µm
Ligand density: 4 – 5 mg Reactive Brown 10 / ml drained medium
Binding capacity: ~3 – 5 mg human serum albumin/ ml gel
pH stability: 3 – 13
Storage: 2 – 8 °C
Supplied as suspension in 0.5M NaCl containing 0.02% thimerosal
References:
Purification and characterization of CMP-N-acetylneuraminic acid hydroxylase from pig submandibular glands. Glycobiology. (1994) 4: 675-83.
Purification and characterization of the nuclear cytidine 5'-monophosphate N-acetylneuraminic acid synthetase from rat liver. J Biol Chem. (1992) 267: 9257-63.
Characterization of sialidase from Entamoaeba hystolitica and possible pathogenic role in amebiasis. Parasitol Res. (2003) 89: 302-7.
Characterisation and immunolocation of an 87 kDa polypeptide associated with UDP-glucuronic acid decarboxylase activity from differentiating tobacco cells (Nicotiana tabacum L.). Phytochemistry. (2002) 61: 771-80.
A 45-kDa midgut glycoprotein from Anopheles albimanus mosquito mediates the killing of trypanosomes. Cell Biochem Funct. (2002) 20: 257-62.
Degradation of aromatics and chloroaromatics by Pseudomonas sp. strain B13: purification and characterization of 3-oxoadipate:succinyl-coenzyme A (CoA) transferase and 3-oxoadipyl-CoA thiolase. J Bacteriol. (2002) 184: 207-15.
Related products:
Red-Separopore® 4B-CL (SKU # 20181095)
Blue-Separopore® 4B-CL (SKU # 20181096)
Reactive Blue 4-Separopore® 4B-CL (SKU #20181097)
Yellow-Separopore® 4B-CL (SKU # 20181098)
Green-Separopore® 4B-CL (SKU # 20181099)
Usage: bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.
Hazmat Shipping: Non-hazardous
Storage: 2-8°C
Brand Name: bioPLUS™, Separopore®
Brown-Separopore® 4B-CL is a dye-ligand affinity matrix consisting of Reactive Brown 10 bound to cross-linked 4% agarose beads. It is used to purify proteins that have distinctive affinity to this dye. Immobilized dyes have been found to bind from 5 to 60 % of the proteins in various crude cell extracts. The affinity for reactive dyes to proteins may be due to substrate /cofactor similarities as well as hydrophobic and ion exchange properties. Some proteins have been found to require the addition of divalent cations for binding to dye resins.
Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.
Technical Specifications:
Ligand: Reactive Brown 10
Matrix: Separopore® 4B-CL (crosslinked agarose beads, 4%)
Particle size range: 52 – 165 µm
Ligand density: 4 – 5 mg Reactive Brown 10 / ml drained medium
Binding capacity: ~3 – 5 mg human serum albumin/ ml gel
pH stability: 3 – 13
Storage: 2 – 8 °C
Supplied as suspension in 0.5M NaCl containing 0.02% thimerosal
References:
Purification and characterization of CMP-N-acetylneuraminic acid hydroxylase from pig submandibular glands. Glycobiology. (1994) 4: 675-83.
Purification and characterization of the nuclear cytidine 5'-monophosphate N-acetylneuraminic acid synthetase from rat liver. J Biol Chem. (1992) 267: 9257-63.
Characterization of sialidase from Entamoaeba hystolitica and possible pathogenic role in amebiasis. Parasitol Res. (2003) 89: 302-7.
Characterisation and immunolocation of an 87 kDa polypeptide associated with UDP-glucuronic acid decarboxylase activity from differentiating tobacco cells (Nicotiana tabacum L.). Phytochemistry. (2002) 61: 771-80.
A 45-kDa midgut glycoprotein from Anopheles albimanus mosquito mediates the killing of trypanosomes. Cell Biochem Funct. (2002) 20: 257-62.
Degradation of aromatics and chloroaromatics by Pseudomonas sp. strain B13: purification and characterization of 3-oxoadipate:succinyl-coenzyme A (CoA) transferase and 3-oxoadipyl-CoA thiolase. J Bacteriol. (2002) 184: 207-15.
Related products:
Red-Separopore® 4B-CL (SKU # 20181095)
Blue-Separopore® 4B-CL (SKU # 20181096)
Reactive Blue 4-Separopore® 4B-CL (SKU #20181097)
Yellow-Separopore® 4B-CL (SKU # 20181098)
Green-Separopore® 4B-CL (SKU # 20181099)
Usage: bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.
Hazmat Shipping: Non-hazardous
Storage: 2-8°C
Brand Name: bioPLUS™, Separopore®
| Is Featured? | No |
|---|
Write Your Own Review