Bst DNA Polymerase

Large Fragment of Bst DNA Polymerase, Exonuclease Minus, is isolated from a Bacillus stearothermophilus. It catalyzes 5’ 3’ synthesis of DNA and lacks 5’ 3’ and 3’ 5’ exonuclease activities.

Source: A recombinant E. coli strain carrying the Bst DNA Polymerase gene (Large fragment).

Advantages & Features
- High Purity: greater than 99% confirmed by SDS PAGE.
- Reverse transcription activity.
- Optimized: Increased activity.
- Strong strand displacement activity.

Specifications
Unit definition:
One unit catalyzes the incorporation of 10 nmol of dNTP into acid-insoluble material in 30 minutes at 65 C in 20 mMTris-HCl pH 8.8, 10 mM (NH4)2SO4,10 mM KCl, 2 mM MgSO4, 0.1% Triton X-100, 30 nM M13mp18 ssDNA, 70 nMM13 sequencing primer(-47) 24-mer 200 µM dGTP, dATP, dTTP, dCTP (a mix of unlabeled and [33P]dCTP) and 0.1 mg/mL BSA.

Includes
- Bst DNA Polymerase (8 U/μL)
- Reaction Buffer (10x)
- 100mM MgSO4 solution

Applications
- Nucleic acid amplification methods.
- Whole genome amplification.
- Multiple displacement amplification.
- DNA Sequencing.

Some uses for this product may require licenses. Canvax does not encourage or support the unauthorized or unlicensed use of patented nucleic acid amplification processes for isothermal amplification, whole genome amplification (WGA), multiple displacement amplification (MDA), and Next Generation sequencing. It is the sole responsibility of the buyer to ensure that use of the product does not infringe the patent rights of third parties.

Shipping & Storage
Shipped in: Gel pack.
Storage: -20 °C (NON Frost-Free Freezer).