BT-plex Human Cytokine Panel I, 12-plex Full Panel
€1,524.00
In stock
SKU
BT-X202005
INTRODUCTION
The BT-plex™ Human Cytokine Panel 1 (12-plex) is a bead-based multiplex assay panel, using magnetic fluorescence-encoded beads for use with regular flow cytometers. The panel allows simultaneous quantification of IL-1ß, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12p70, IL-17A, IFN-α2, IFN-γ, and TNF-α, or any combination of the key cytokines involved in major immune responses. The kit provides ultra-sensitive detection (fg/mL) and is more robust in analytical performance than similar multiplex assays on the market. This panel has been tested for use on cell culture supernatant, serum, plasma, and other body fluid samples.
The BT-plex™ Human Cytokine Panel 1 is designed to allow flexible customization. Users can select any combination of the targets within the panel. Please email us (info@emelcabio.com) for more product information or call us (+31.618 407 397) to discuss your specific needs.
Special features:
Magnetic beads allows easier handling and automation
Better assay sensitivities with most targets at low fg/mL level
Small sample volume, only 10 µL required
Two levels of Control Samples included when requested
Large reaction volume (50 µL beads and detection antibody) allows better reproducibility
Flexibility with either V-, U-, F-bottom, Filter plates or microfuge and mini FACS tubes
More economical with lower cost
ASSAY PRINCIPLES
BT-plexTM bead-based kits use the same principle as typical sandwich immunoassays. Magnetic beads are differentiated by a combination of bead size and internal fluorescence intensities. Each bead set has a functional surface and is chemically conjugated with a specific capture molecule (e.g., antibody or binding protein) on the surface of the bead specific for a given analyte. When a selected group of beads with different Bead IDs are mixed and incubated with a sample, each target analyte in the sample will bind to its specific capture beads. A mixture of biotinylated detection antibodies specific to the targets are added, each detection antibody in the cocktail will bind to its specific analyte bound on the capture beads, thus forming capture bead-analyte-detection antibody sandwiches. Streptavidin-phycoerythrin (SA-PE) subsequently binds to the biotin group on the detection antibodies, providing a fluorescent signal with intensities in proportion to the quantity of bound analytes. With a flow cytometer reader, analyte-specific populations can be segregated and phycoerythrin fluorescent signal can be quantified. The concentration of a particular analyte is determined using a standard curve generated in the same assay.
STANDARD RANGE: 2000 – 0.5 pg/ml
SENSITIVITY
The assay sensitivity (minimum detectable concentration, MinDC) is the theoretical limit of detection calculated using the BT-plex Data Analysis Software by applying a 5-parameter curve fitting algorithm with weighting. The assay sensitivity data below is for reference use only.
Sensitivity MinDC (pg/mL)
IL-1ß 0.10
IL-2 0.02
IL-4 0.05
IL-5 0.05
IL-6 0.04
IL-8 0.08
IL-10 0.04
IL-12p70 0.04
IL-17A 0.22
IFN-α2 0.06
IFN-gamma 0.01
TNF-α 1.62
Components Quantity Volume Item#
Premixed Beads 1 Bottle 6.0 mL X202005-01
Detection Antibody 1 Bottle 6.0 mL X202005-02
Standard, Lyophilized 1 Bottle _ X202005-03
BT-plex SA-PE Solution 1 Bottle 6.0 mL X20205-04
BT-plex Assay Buffer 2 Bottles 25 mL BTAB01
BT-plex 20X Wash Buffer 1 Bottle 25 mL BTWB01
Control 1, Lyophilized 1 Bottle _ X202005-05
Control 2, Lyophilized 1 Bottle _ X202005-06
V-Bottom Plate 1 Plate _ NA
Adhesive Plate Sealer 2 Sheets NA NA
Kit Instruction 1 Copy NA NA
The BT-plex™ Human Cytokine Panel 1 (12-plex) is a bead-based multiplex assay panel, using magnetic fluorescence-encoded beads for use with regular flow cytometers. The panel allows simultaneous quantification of IL-1ß, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12p70, IL-17A, IFN-α2, IFN-γ, and TNF-α, or any combination of the key cytokines involved in major immune responses. The kit provides ultra-sensitive detection (fg/mL) and is more robust in analytical performance than similar multiplex assays on the market. This panel has been tested for use on cell culture supernatant, serum, plasma, and other body fluid samples.
The BT-plex™ Human Cytokine Panel 1 is designed to allow flexible customization. Users can select any combination of the targets within the panel. Please email us (info@emelcabio.com) for more product information or call us (+31.618 407 397) to discuss your specific needs.
Special features:
Magnetic beads allows easier handling and automation
Better assay sensitivities with most targets at low fg/mL level
Small sample volume, only 10 µL required
Two levels of Control Samples included when requested
Large reaction volume (50 µL beads and detection antibody) allows better reproducibility
Flexibility with either V-, U-, F-bottom, Filter plates or microfuge and mini FACS tubes
More economical with lower cost
ASSAY PRINCIPLES
BT-plexTM bead-based kits use the same principle as typical sandwich immunoassays. Magnetic beads are differentiated by a combination of bead size and internal fluorescence intensities. Each bead set has a functional surface and is chemically conjugated with a specific capture molecule (e.g., antibody or binding protein) on the surface of the bead specific for a given analyte. When a selected group of beads with different Bead IDs are mixed and incubated with a sample, each target analyte in the sample will bind to its specific capture beads. A mixture of biotinylated detection antibodies specific to the targets are added, each detection antibody in the cocktail will bind to its specific analyte bound on the capture beads, thus forming capture bead-analyte-detection antibody sandwiches. Streptavidin-phycoerythrin (SA-PE) subsequently binds to the biotin group on the detection antibodies, providing a fluorescent signal with intensities in proportion to the quantity of bound analytes. With a flow cytometer reader, analyte-specific populations can be segregated and phycoerythrin fluorescent signal can be quantified. The concentration of a particular analyte is determined using a standard curve generated in the same assay.
STANDARD RANGE: 2000 – 0.5 pg/ml
SENSITIVITY
The assay sensitivity (minimum detectable concentration, MinDC) is the theoretical limit of detection calculated using the BT-plex Data Analysis Software by applying a 5-parameter curve fitting algorithm with weighting. The assay sensitivity data below is for reference use only.
Sensitivity MinDC (pg/mL)
IL-1ß 0.10
IL-2 0.02
IL-4 0.05
IL-5 0.05
IL-6 0.04
IL-8 0.08
IL-10 0.04
IL-12p70 0.04
IL-17A 0.22
IFN-α2 0.06
IFN-gamma 0.01
TNF-α 1.62
Components Quantity Volume Item#
Premixed Beads 1 Bottle 6.0 mL X202005-01
Detection Antibody 1 Bottle 6.0 mL X202005-02
Standard, Lyophilized 1 Bottle _ X202005-03
BT-plex SA-PE Solution 1 Bottle 6.0 mL X20205-04
BT-plex Assay Buffer 2 Bottles 25 mL BTAB01
BT-plex 20X Wash Buffer 1 Bottle 25 mL BTWB01
Control 1, Lyophilized 1 Bottle _ X202005-05
Control 2, Lyophilized 1 Bottle _ X202005-06
V-Bottom Plate 1 Plate _ NA
Adhesive Plate Sealer 2 Sheets NA NA
Kit Instruction 1 Copy NA NA
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