Bub1 polyclonal, anti-human, mouse

Protein Family: Enzymes

Pathway and Disease: Signaling Molecules and Interaction

Description:
Bub1 was initially identified as the mammalian homolog to the yeast protein through a cDNA screen. Bub1 encodes a kinase involved in the formation of the spindle checkpoint, an important mechanism that ensures high fidelity mitotic chromosome segregation. It is thought to be required for assembly of a functional inner centromere, sister chromatid cohesion via targeting of the Shugoshim protein and metaphase congression. Bub1 functions by phosphorylating cdc20, a member of the mitotic checkpoint complex and activating the spindle checkpoint. A related protein kinase Bub3 interacts with Bub1 and targets it to kinetochores prior to chromosome alignment. Mutations in bub1 have been associated with aneuploidy and several forms of cancer.

Alternate Names: Bub1 , Budding uninhibited by benzimidazoles 1

Application Notes: E: 1:500-1:1,000; WB: 1:100-1:500; ICC: 1:100-1:500

Accession No.: O43683

Antigen: KLH-conjugated synthetic peptide encompassing a sequence within human Bub1.

Format: Each vial contains 0.1 ml IgG in PBS pH 7.4 with 0.02% sodium azide. Antibody was purified by immunogen affinity chromatography.

Storage:
Store at -20°C. Minimize freeze-thaw cycles. Product is guaranteed one year from the date of shipment.