Butyl Separopore® 4B-CL
€0.00
In stock
SKU
BW-20181102
Application: Butyl-Separopore® 4B-CL is a protein separation media for hydrophobic interaction chromatography. Substances are separated on the basis of their different hydrophobicities.
Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.
Technical Specifications:
Ligand: Butyl group
Matrix: Separopore® 4B-CL (crosslinked agarose beads, 4%)
Particle size range: 52 – 165 μm
Molecular weight range: 6 x 104 – 2 x 107
Ligand density: 10 – 14 μmol butyl groups / ml drained gel
Matrix activation: Epoxy
Matrix spacer: 11 atoms
Capacity: ~ 15 mg BSA / ml drained gel
pH stability: 4 – 9
Flow Specifications: 70 – 140 cm / h
Supplied as suspension in 0.5M NaCl containing 0.02% thimerosal
References:
N-acetyl-D-neuraminic acid lyase generates the sialic acid for colominic acid biosynthesis in Escherichia coli K1. Biochem J. (1996) 317: 157-65.
High level expression, purification and activation of human dipeptidyl peptidase I from mammalian cells. Protein Expr Purif. (2011) 76: 59-64.
Purification, immobilization, and characterization of a specific lipase from Staphylococcus warneri EX17 by enzyme fractionating via adsorption on different hydrophobic supports. Biotechnol Prog. (2011) 27: 717-23.
Efficient separation of homologous alpha-lactalbumin from transgenic bovine milk using optimized hydrophobic interaction chromatography. J Chromatogr A. (2010) 1217: 3668-73.
Expression and purification of canine granulocyte colony-stimulating factor (cG-CSF). Vet Immunol Immunopathol. (2009) 130: 221-5.
Separation of product associating E. coli host cell proteins OppA and DppA from recombinant apolipoprotein A-I(Milano) in an industrial HIC unit operation. Biotechnol Prog. (2009) 25: 446-53.
Angiotensin I retention behavior on Butyl-Sepharose under linear loading chromatographic conditions. J Chromatogr A. (2009) 1216: 2332-8.
Purification and characterization of a major collagenase from Streptomyces parvulus. Biosci Biotechnol Biochem. (2009) 73: 21-8.
Purification and properties of a chitinase from Penicillium sp. LYG 0704. Protein Expr Purif. (2009) 65: 244-50.
Optimization of hydrophobic interaction chromatography using a mathematical model of elution curves of a protein mixture. J Mol Recognit. (2009) 22: 110-20.
Process development for production of human granulocyte-colony stimulating factor by high cell density cultivation of recombinant Escherichia coli. J Ind Microbiol Biotechnol. (2008) 35: 1643-50.
Advancement in the modeling of pressure-flow for the guidance of development and scale-up of commercial-scale biopharmaceutical chromatography. J Chromatogr A. (2008) 1190: 127-40.
Theory and Application: of refractive index-based optical microscopy to measure protein mass transfer in spherical adsorbent particles. J Chromatogr A. (2008) 1188: 242-54.
Isolation of soybean protein P34 from oil bodies using hydrophobic interaction chromatography. BMC Biotechnol. (2008) 8: 27.
Related products:
Octyl-Separopore® 4B-CL (SKU # 20181073)
Phenyl-Separopore® 4B-CL (SKU # 20181074)
Usage: bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.
Hazmat Shipping: Non-hazardous
Storage: 2-8°C
Brand Name: bioPLUS™, Separopore®
Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.
Technical Specifications:
Ligand: Butyl group
Matrix: Separopore® 4B-CL (crosslinked agarose beads, 4%)
Particle size range: 52 – 165 μm
Molecular weight range: 6 x 104 – 2 x 107
Ligand density: 10 – 14 μmol butyl groups / ml drained gel
Matrix activation: Epoxy
Matrix spacer: 11 atoms
Capacity: ~ 15 mg BSA / ml drained gel
pH stability: 4 – 9
Flow Specifications: 70 – 140 cm / h
Supplied as suspension in 0.5M NaCl containing 0.02% thimerosal
References:
N-acetyl-D-neuraminic acid lyase generates the sialic acid for colominic acid biosynthesis in Escherichia coli K1. Biochem J. (1996) 317: 157-65.
High level expression, purification and activation of human dipeptidyl peptidase I from mammalian cells. Protein Expr Purif. (2011) 76: 59-64.
Purification, immobilization, and characterization of a specific lipase from Staphylococcus warneri EX17 by enzyme fractionating via adsorption on different hydrophobic supports. Biotechnol Prog. (2011) 27: 717-23.
Efficient separation of homologous alpha-lactalbumin from transgenic bovine milk using optimized hydrophobic interaction chromatography. J Chromatogr A. (2010) 1217: 3668-73.
Expression and purification of canine granulocyte colony-stimulating factor (cG-CSF). Vet Immunol Immunopathol. (2009) 130: 221-5.
Separation of product associating E. coli host cell proteins OppA and DppA from recombinant apolipoprotein A-I(Milano) in an industrial HIC unit operation. Biotechnol Prog. (2009) 25: 446-53.
Angiotensin I retention behavior on Butyl-Sepharose under linear loading chromatographic conditions. J Chromatogr A. (2009) 1216: 2332-8.
Purification and characterization of a major collagenase from Streptomyces parvulus. Biosci Biotechnol Biochem. (2009) 73: 21-8.
Purification and properties of a chitinase from Penicillium sp. LYG 0704. Protein Expr Purif. (2009) 65: 244-50.
Optimization of hydrophobic interaction chromatography using a mathematical model of elution curves of a protein mixture. J Mol Recognit. (2009) 22: 110-20.
Process development for production of human granulocyte-colony stimulating factor by high cell density cultivation of recombinant Escherichia coli. J Ind Microbiol Biotechnol. (2008) 35: 1643-50.
Advancement in the modeling of pressure-flow for the guidance of development and scale-up of commercial-scale biopharmaceutical chromatography. J Chromatogr A. (2008) 1190: 127-40.
Theory and Application: of refractive index-based optical microscopy to measure protein mass transfer in spherical adsorbent particles. J Chromatogr A. (2008) 1188: 242-54.
Isolation of soybean protein P34 from oil bodies using hydrophobic interaction chromatography. BMC Biotechnol. (2008) 8: 27.
Related products:
Octyl-Separopore® 4B-CL (SKU # 20181073)
Phenyl-Separopore® 4B-CL (SKU # 20181074)
Usage: bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.
Hazmat Shipping: Non-hazardous
Storage: 2-8°C
Brand Name: bioPLUS™, Separopore®
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