CA19-9, Sialyl Lewis a (clone 121SLE), anti-human

Source:
A BALB/c mouse was immunized with ten precipitin lines obtained after immunodiffusion using mAb NS19-9 and mucins isolated from an ovarian cyst of a 0Lea+b- patient.
Fusion partner: Sp2/0.

Specifications:
121SLE reacts with CA19-9 (>400 kDa) or sialyl Lea structure, which is synthesized from type 1 blood group precursor chains and is present in individuals expressing the Lea and/or Lebblood group antigens. 121SLE also binds to some extend to the afuco version of SLea (LSTa; CA50). In normal tissues, CA19-9 is present in ductal epithelium of the breast, kidney, salivary, gland and sweat glands. Its expression is greatly enhanced in serum as well as in the majority of tumor cells in gastrointestinal (GI) carcinomas, including adenocarcinomas of the stomach, intestine and pancreas. 121SLE was typed in the ISOBM TD-6 workshop.

Species reactivity:
Positive: human.

Applications:
121SLE is excellent for staining of CA19-9 on paraffin sections. No pre-treatment is required. 121SLE is moderately suitable for ELISA. Good results were obtained when B25.10 was used as solid phase antibody.
ELISA: +
Flow cytometry: +
Frozen sections: +
Paraffin sections: +

Positive control: Stomach or colon carcinoma.

References:
- Blood transfusion and Immunohaematology, Ph. Rouger, D Anstee and Ch. Salmon(Eds). Amette,France 30 (5): 353-720, (1987).
- Rye PD. et al, Tumor Biol19 (5): 390-420, (1998).
- Christopher MG. et al, Cytojournal8: 7 (2011).