CALIBRATOR BKV CE-IVD
€525.00
In stock
SKU
RealCycler BKVQ
Catalog Number: RealCycler BKVQ
Quantity: 225 μl
Quantity: 225 μl
RealCycler products allow the quantification of pathogens when they are used in combination with the corresponding calibrator kit.
Calibrator kits allow to obtain a calibration curve which is used as reference to quantify the pathogen. This curve is calculated only once, and can be used to quantify subsequent work series.
Calibrator kits consist of various quantified dilutions of a microorganism. They have to be processed exactly as the clinical samples, using the same DNA / RNA extraction system, the same working volumes and the same version of the corresponding RealCycler product.
Calibrator kits can be used in compatible real-time PCR instruments (consult compatibility) and regardless of the extraction method. Optionally, an in-house calibrator can be used to quantify clinical samples using RealCycler products.
The software Visor RealCycler v2 allows automatic and simultaneous quantification of all the pathogens present in the sample.
1 Intended use
RealCycler BKVQ is a kit of reagents which allows the preparation of a calibration curve for the quantification of polyomavirus BK (BKV) DNA in clinical samples.
2 Principle of the test
The polymerase chain reaction (PCR) is based on the amplification of a specific region of the DNA/RNA by using complementary primers to the target sequence. Real-time PCR uses marked probes with fluorophores that emit fluorescence in the case of amplification. The cycle of the PCR protocol in which appears significant fluorescence is proportional to the DNA/RNA quantity present in the sample. This value is called Cycle Treshold (Ct).
The calibration curve is used to measure the concentration of a substance by comparing it with known concentration substances obtained by dilution.
3 Contents
RealCycler BKVQ system includes 4 vials containing 4 BKV standards with serial concentrations expressed in copy per μL units (copies/μL), which concentrations vary from 1000 copies/μL to 1 copy/μL.
The 4 quantification standards are ready to use without adding or rebuilding any component.
Calibrator kits allow to obtain a calibration curve which is used as reference to quantify the pathogen. This curve is calculated only once, and can be used to quantify subsequent work series.
Calibrator kits consist of various quantified dilutions of a microorganism. They have to be processed exactly as the clinical samples, using the same DNA / RNA extraction system, the same working volumes and the same version of the corresponding RealCycler product.
Calibrator kits can be used in compatible real-time PCR instruments (consult compatibility) and regardless of the extraction method. Optionally, an in-house calibrator can be used to quantify clinical samples using RealCycler products.
The software Visor RealCycler v2 allows automatic and simultaneous quantification of all the pathogens present in the sample.
1 Intended use
RealCycler BKVQ is a kit of reagents which allows the preparation of a calibration curve for the quantification of polyomavirus BK (BKV) DNA in clinical samples.
2 Principle of the test
The polymerase chain reaction (PCR) is based on the amplification of a specific region of the DNA/RNA by using complementary primers to the target sequence. Real-time PCR uses marked probes with fluorophores that emit fluorescence in the case of amplification. The cycle of the PCR protocol in which appears significant fluorescence is proportional to the DNA/RNA quantity present in the sample. This value is called Cycle Treshold (Ct).
The calibration curve is used to measure the concentration of a substance by comparing it with known concentration substances obtained by dilution.
3 Contents
RealCycler BKVQ system includes 4 vials containing 4 BKV standards with serial concentrations expressed in copy per μL units (copies/μL), which concentrations vary from 1000 copies/μL to 1 copy/μL.
The 4 quantification standards are ready to use without adding or rebuilding any component.
| Is Featured? | No |
|---|
Write Your Own Review