Calmodulin Red Separopore® 4B Microbeads viewIP™
€0.00
In stock
SKU
BW-20181104
Application:
Red-colored agarose matrix contains immobilized bovine calmodulin for convenient affinity capture (pull-down) target molecules. viewIP™ Calmodulin Red-Separopore® 4B-CL greatly reduce the risk of pellet loss. Comparable performance to non-colored affinity gels, but allows the user to easily differentiate pellet from supernatant. This results in greater consistency and more accurate data due to less sample losses.
viewIP™ Calmodulin Red-Separopore® 4B-CL is used for convenient affinity purification of calmodulin-binding proteins including calmodulin-regulated proteins in eukaryotic cells and recombinant proteins containing calmodulin-binding peptide fusion tag.
Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.
Technical Specifications:
Ligand: Calmodulin (bovine brain)
Matrix: Separopore® 4B-CL (crosslinked agarose beads, 4%)
Ligand coupling method: CNBr activation
Particle size range: 52 – 165 µm
Exclusion limit: approximately 2 x 107
pH stability: 3 – 9 (for use)
Supplied as 50% suspension in PBS, containing 50% glycerol and 0.02% sodium azide
Storage: 2 – 8 °C
References:
Arabidopsis ATPase family gene 1-like protein 1 is a calmodulin-binding AAA+-ATPase with a dual localization in chloroplasts and mitochondria. FEBS J. (2009) 276: 3870-80.
Calpain-mediated androgen receptor breakdown in apoptotic prostate cancer cells. J Cell Physiol. (2008) 217: 569-76.
Analysis of the interaction of influenza virus polymerase complex with human cell factors. Proteomics. (2008) 8: 2077-88.
Structural analyses of the ankyrin repeat domain of TRPV6 and related TRPV ion channels. Biochemistry. (2008) 47: 2476-84.
Oligomerization of the influenza virus polymerase complex in vivo. J Gen Virol. (2008) 89: 520-4.
Protein-tyrosine kinase CAKbeta/PYK2 is activated by binding Ca2+/calmodulin to FERM F2 alpha2 helix and thus forming its dimer. Biochem J. (2008) 410: 513-23.
Purification of recombinant membrane proteins tagged with calmodulin-binding domains by affinity chromatography on calmodulin-agarose: example of nicotinamide nucleotide transhydrogenase. Nat Protoc. (2007) 2: 198-202.
Identification of the divergent calmodulin binding motif in yeast Ssb1/Hsp75 protein and in other HSP70 family members. Braz J Med Biol Res. (2006) 39: 1399-408.
Calcium regulation of chloroplast protein translocation is mediated by calmodulin binding to Tic32. Proc Natl Acad Sci U S A. (2006) 103: 16051-6.
Isolation, characterization, and bioinformatic analysis of calmodulin-binding protein cmbB reveals a novel tandem IP22 repeat common to many Dictyostelium and Mimivirus proteins. Biochem Biophys Res Commun. (2006) 346: 879-88.
Purification of Mg2+-dependent phosphatidate phosphohydrolase from rat liver: new steps and aspects. Biol Chem. (2005) 386: 1197-201.
Identification and characterization of a novel alpha-kinase with a von Willebrand factor A-like motif localized to the contractile vacuole and Golgi complex in Dictyostelium discoideum. Mol Biol Cell. (2005) 16: 2248-62.
Effect of apocalmodulin on recombinant human brain glutamic acid decarboxylase. J Neurochem. (2005) 92: 739-48.
Myristoyl moiety of HIV Nef is involved in regulation of the interaction with calmodulin in vivo. Protein Sci. (2005) 14: 494-503.
Calmodulin binds to and inhibits the activity of phosphoglycerate kinase. Biochim Biophys Acta. (2004) 1693: 177-83.
Calmodulin interacts with the V2 vasopressin receptor: elimination of binding to the C terminus also eliminates arginine vasopressin-stimulated elevation of intracellular calcium. J Biol Chem. (2004) 279: 46969-80.
Direct involvement of protein myristoylation in myristoylated alanine-rich C kinase substrate (MARCKS)-calmodulin interaction. J Biol Chem. (2003) 278: 48898-902.
A conserved calcineurin-binding motif in human T lymphotropic virus type 1 p12I functions to modulate nuclear factor of activated T cell activation. J Biol Chem. (2003) 278: 15550-7.
Evidence for a MARCKS-PKCalpha complex in skeletal muscle. Int J Biochem Cell Biol. (2001) 33: 711-21.
Alternative splicing of a novel diacylglycerol kinase in tomato leads to a calmodulin-binding isoform. Plant J. (2000) 24: 317-26.
Regional distribution, ontogeny, purification, and characterization of the Ca2+-independent phospholipase A2 from rat brain. J Neurochem. (1999) 73: 1278-87.
A calcium/calmodulin-dependent nitric oxide synthase, NMDAR2/3 receptor subunits, and glutamate in the CNS of the cuttlefish Sepia officinalis: localization in specific neural pathways controlling the inking system. J Neurochem. (1999) 73: 1254-63.
Nitric oxide synthase from bovine pancreas: purification and characterization. Arch Pharm Res. (1998) 21: 128-34.
Inhibition of Ca2+/calmodulin-dependent phosphatise activity by regucalcin in rat liver cytosol: involvement of calmodulin binding. J Cell Biochem. (1998) 71: 140-8.
Ca2+/calmodulin dependent protein kinase from Mycobacterium smegmatis ATCC 607. Mol Cell Biochem. (1998) 183: 183-91.
A maize FK506-sensitive immunophilin, mzFKBP-66, is a peptidylproline cis-trans-isomerase that interacts with calmodulin and a 36-kDa cytoplasmic protein. Planta. (1998) 205: 121-31.
Purification of the Plasma Membrane Ca2+-ATPase from Radish Seedlings by Calmodulin-Agarose Affinity Chromatography. Plant Physiol. (1998) 116: 845-51.
Smad proteins physically interact with calmodulin. J Biol Chem. (1998) 273: 677-80.
Proliferative stimulation of lymphocytes by calmodulin binding proteins isolated from amniotic fluid. Indian J Med Res. (1997) 106: 236-41.
Calcium-induced activation of the rat vascular myocyte Na+/H+ exchanger isoform-1. Metabolism. (1997) 46: 250-6.
The regulatory Ser262 of microtubule-associated protein tau is phosphorylated by phosphorylase kinase. J Biol Chem. (1997) 272: 1777-85.
HER-2/c-erbB2 is phosphorylated by calmodulin-dependent protein kinase II on a single site in the cytoplasmic tail at threonine-1172. Oncogene. (1996) 12: 2725-30.
Cyclic nucleotide phosphodiesterase (PDE) isoenzymes in the human detrusor smooth muscle. I. Identification and characterization. Urol Res. (1996) 24: 123-8.
Identification of a new microtubule-interacting protein Mip-90. Eur J Cell Biol. (1995) 67: 158-69.
Biochemical and calmodulin binding properties of estrogen receptor binding cyclophilin expressed in Escherichia coli. Biochem Biophys Res Commun. (1995) 209: 117-25.
The 68 kDa calmodulin-binding protein is tightly associated with the multiprotein DNA polymerase alpha-primase complex in HeLa cells. Biochemistry. (1995) 34: 3878-83.
Characterization of the plasma-membrane calcium pump from Trypanosoma cruzi. Biochem J. (1995) 306: 299-303.
Autoradiographic distribution and characteristics of high- and low-affinity polyamine-sensitive [3H]ifenprodil sites in the rat brain: possible relationship to NMDAR2B receptors and calmodulin. J Neurochem. (1994) 63: 2248-58.
Activation of calcium and calmodulin dependent protein kinase II during stimulation of insulin secretion. Cell Calcium. (1994) 16: 137-50.
Separation and characterization of two Mg2+-ATPase activities from the human erythrocyte membrane. Arch Biochem Biophys. (1994) 312: 272-7.
Characterization of a novel high molecular mass protein with peptidase activity purified from the human erythrocyte membrane by calmodulin affinity chromatography. J Biol Chem. (1994) 269: 10016-21.
Purification and characterization of the high molecule weight microtubule associated proteins from neonatal rat brain. Mol Cell Biochem. (1994) 131: 105-13.
A simple and efficient method for the purification of seminal plasmin, an antimicrobial protein from bovine seminal plasma. Prep Biochem. (1994) 24: 15-24.
Thyroid hormone stimulates release of calmodulin-enhancing activity from human erythrocyte membranes in vitro. Clin Sci (Lond). (1993) 84: 217-23.
A single step purification for recombinant proteins. Characterization of a microtubule associated protein (MAP 2) fragment which associates with the type II cAMP-dependent protein kinase. FEBS Lett. (1992) 302: 274-8.
Ca2+/calmodulin-dependent protein kinase II from hen brain. Purification and characterization. Biochem Pharmacol. (1992) 43: 1975-88.
A purification strategy for inositol 1,4,5-trisphosphate 3-kinase from rat liver based upon heparin interaction chromatography. Cell Signal. (1992) 4: 303-12.
Isolation and characterization of a new Ca2+/calmodulin-dependent protein kinase from isoproterenol-stimulated proliferating rat parotid acinar cells. Biochem Cell Biol. (1992) 70: 250-5.
Isolation and characterization of catalytic and calmodulin-binding domains of Bordetella pertussis adenylate cyclase. Eur J Biochem. (1991) 196: 469-74.
Purification of a soluble isoform of guanylyl cyclase-activating-factor synthase. Proc Natl Acad Sci U S A. (1991) 88: 365-9.
Evidence for a specific recognition site for tiflucarbine on calmodulin. Eur J Pharmacol. (1990) 189: 411-8.
Purification and characterization of a calcium-calmodulin-dependent phospholamban kinase from canine myocardium. Biochemistry. (1989) 28: 5909-16.
The effect of calmodulin on the interaction of carbodiimides with the purified human erythrocyte (Ca2+ + Mg2+)-ATPase. Biochim Biophys Acta. (1988) 945: 33-40.
Purification of D-myo-inositol 1,4,5-trisphosphate 3-kinase from rat brain. J Biol Chem. (1988) 263: 7465-71.
Characterization of the calmodulin-binding sites of muscle phosphofructokinase and comparison with known calmodulin-binding domains. J Biol Chem. (1987) 262: 9454-62.
Isolation and properties of the active gamma subunit of phosphorylase kinase. J Biol Chem. (1986) 261: 4732-7.
Calmodulin activation of cyclic AMP phosphodiesterase in the B16 mouse melanoma. Biochem J. (1984) 219: 941-6.
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Usage : bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.
Hazmat Shipping : Non-hazardous
Storage : 2-8°C
Brand Name : bioPLUS™, Separopore®, ViewIP™
Red-colored agarose matrix contains immobilized bovine calmodulin for convenient affinity capture (pull-down) target molecules. viewIP™ Calmodulin Red-Separopore® 4B-CL greatly reduce the risk of pellet loss. Comparable performance to non-colored affinity gels, but allows the user to easily differentiate pellet from supernatant. This results in greater consistency and more accurate data due to less sample losses.
viewIP™ Calmodulin Red-Separopore® 4B-CL is used for convenient affinity purification of calmodulin-binding proteins including calmodulin-regulated proteins in eukaryotic cells and recombinant proteins containing calmodulin-binding peptide fusion tag.
Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.
Technical Specifications:
Ligand: Calmodulin (bovine brain)
Matrix: Separopore® 4B-CL (crosslinked agarose beads, 4%)
Ligand coupling method: CNBr activation
Particle size range: 52 – 165 µm
Exclusion limit: approximately 2 x 107
pH stability: 3 – 9 (for use)
Supplied as 50% suspension in PBS, containing 50% glycerol and 0.02% sodium azide
Storage: 2 – 8 °C
References:
Arabidopsis ATPase family gene 1-like protein 1 is a calmodulin-binding AAA+-ATPase with a dual localization in chloroplasts and mitochondria. FEBS J. (2009) 276: 3870-80.
Calpain-mediated androgen receptor breakdown in apoptotic prostate cancer cells. J Cell Physiol. (2008) 217: 569-76.
Analysis of the interaction of influenza virus polymerase complex with human cell factors. Proteomics. (2008) 8: 2077-88.
Structural analyses of the ankyrin repeat domain of TRPV6 and related TRPV ion channels. Biochemistry. (2008) 47: 2476-84.
Oligomerization of the influenza virus polymerase complex in vivo. J Gen Virol. (2008) 89: 520-4.
Protein-tyrosine kinase CAKbeta/PYK2 is activated by binding Ca2+/calmodulin to FERM F2 alpha2 helix and thus forming its dimer. Biochem J. (2008) 410: 513-23.
Purification of recombinant membrane proteins tagged with calmodulin-binding domains by affinity chromatography on calmodulin-agarose: example of nicotinamide nucleotide transhydrogenase. Nat Protoc. (2007) 2: 198-202.
Identification of the divergent calmodulin binding motif in yeast Ssb1/Hsp75 protein and in other HSP70 family members. Braz J Med Biol Res. (2006) 39: 1399-408.
Calcium regulation of chloroplast protein translocation is mediated by calmodulin binding to Tic32. Proc Natl Acad Sci U S A. (2006) 103: 16051-6.
Isolation, characterization, and bioinformatic analysis of calmodulin-binding protein cmbB reveals a novel tandem IP22 repeat common to many Dictyostelium and Mimivirus proteins. Biochem Biophys Res Commun. (2006) 346: 879-88.
Purification of Mg2+-dependent phosphatidate phosphohydrolase from rat liver: new steps and aspects. Biol Chem. (2005) 386: 1197-201.
Identification and characterization of a novel alpha-kinase with a von Willebrand factor A-like motif localized to the contractile vacuole and Golgi complex in Dictyostelium discoideum. Mol Biol Cell. (2005) 16: 2248-62.
Effect of apocalmodulin on recombinant human brain glutamic acid decarboxylase. J Neurochem. (2005) 92: 739-48.
Myristoyl moiety of HIV Nef is involved in regulation of the interaction with calmodulin in vivo. Protein Sci. (2005) 14: 494-503.
Calmodulin binds to and inhibits the activity of phosphoglycerate kinase. Biochim Biophys Acta. (2004) 1693: 177-83.
Calmodulin interacts with the V2 vasopressin receptor: elimination of binding to the C terminus also eliminates arginine vasopressin-stimulated elevation of intracellular calcium. J Biol Chem. (2004) 279: 46969-80.
Direct involvement of protein myristoylation in myristoylated alanine-rich C kinase substrate (MARCKS)-calmodulin interaction. J Biol Chem. (2003) 278: 48898-902.
A conserved calcineurin-binding motif in human T lymphotropic virus type 1 p12I functions to modulate nuclear factor of activated T cell activation. J Biol Chem. (2003) 278: 15550-7.
Evidence for a MARCKS-PKCalpha complex in skeletal muscle. Int J Biochem Cell Biol. (2001) 33: 711-21.
Alternative splicing of a novel diacylglycerol kinase in tomato leads to a calmodulin-binding isoform. Plant J. (2000) 24: 317-26.
Regional distribution, ontogeny, purification, and characterization of the Ca2+-independent phospholipase A2 from rat brain. J Neurochem. (1999) 73: 1278-87.
A calcium/calmodulin-dependent nitric oxide synthase, NMDAR2/3 receptor subunits, and glutamate in the CNS of the cuttlefish Sepia officinalis: localization in specific neural pathways controlling the inking system. J Neurochem. (1999) 73: 1254-63.
Nitric oxide synthase from bovine pancreas: purification and characterization. Arch Pharm Res. (1998) 21: 128-34.
Inhibition of Ca2+/calmodulin-dependent phosphatise activity by regucalcin in rat liver cytosol: involvement of calmodulin binding. J Cell Biochem. (1998) 71: 140-8.
Ca2+/calmodulin dependent protein kinase from Mycobacterium smegmatis ATCC 607. Mol Cell Biochem. (1998) 183: 183-91.
A maize FK506-sensitive immunophilin, mzFKBP-66, is a peptidylproline cis-trans-isomerase that interacts with calmodulin and a 36-kDa cytoplasmic protein. Planta. (1998) 205: 121-31.
Purification of the Plasma Membrane Ca2+-ATPase from Radish Seedlings by Calmodulin-Agarose Affinity Chromatography. Plant Physiol. (1998) 116: 845-51.
Smad proteins physically interact with calmodulin. J Biol Chem. (1998) 273: 677-80.
Proliferative stimulation of lymphocytes by calmodulin binding proteins isolated from amniotic fluid. Indian J Med Res. (1997) 106: 236-41.
Calcium-induced activation of the rat vascular myocyte Na+/H+ exchanger isoform-1. Metabolism. (1997) 46: 250-6.
The regulatory Ser262 of microtubule-associated protein tau is phosphorylated by phosphorylase kinase. J Biol Chem. (1997) 272: 1777-85.
HER-2/c-erbB2 is phosphorylated by calmodulin-dependent protein kinase II on a single site in the cytoplasmic tail at threonine-1172. Oncogene. (1996) 12: 2725-30.
Cyclic nucleotide phosphodiesterase (PDE) isoenzymes in the human detrusor smooth muscle. I. Identification and characterization. Urol Res. (1996) 24: 123-8.
Identification of a new microtubule-interacting protein Mip-90. Eur J Cell Biol. (1995) 67: 158-69.
Biochemical and calmodulin binding properties of estrogen receptor binding cyclophilin expressed in Escherichia coli. Biochem Biophys Res Commun. (1995) 209: 117-25.
The 68 kDa calmodulin-binding protein is tightly associated with the multiprotein DNA polymerase alpha-primase complex in HeLa cells. Biochemistry. (1995) 34: 3878-83.
Characterization of the plasma-membrane calcium pump from Trypanosoma cruzi. Biochem J. (1995) 306: 299-303.
Autoradiographic distribution and characteristics of high- and low-affinity polyamine-sensitive [3H]ifenprodil sites in the rat brain: possible relationship to NMDAR2B receptors and calmodulin. J Neurochem. (1994) 63: 2248-58.
Activation of calcium and calmodulin dependent protein kinase II during stimulation of insulin secretion. Cell Calcium. (1994) 16: 137-50.
Separation and characterization of two Mg2+-ATPase activities from the human erythrocyte membrane. Arch Biochem Biophys. (1994) 312: 272-7.
Characterization of a novel high molecular mass protein with peptidase activity purified from the human erythrocyte membrane by calmodulin affinity chromatography. J Biol Chem. (1994) 269: 10016-21.
Purification and characterization of the high molecule weight microtubule associated proteins from neonatal rat brain. Mol Cell Biochem. (1994) 131: 105-13.
A simple and efficient method for the purification of seminal plasmin, an antimicrobial protein from bovine seminal plasma. Prep Biochem. (1994) 24: 15-24.
Thyroid hormone stimulates release of calmodulin-enhancing activity from human erythrocyte membranes in vitro. Clin Sci (Lond). (1993) 84: 217-23.
A single step purification for recombinant proteins. Characterization of a microtubule associated protein (MAP 2) fragment which associates with the type II cAMP-dependent protein kinase. FEBS Lett. (1992) 302: 274-8.
Ca2+/calmodulin-dependent protein kinase II from hen brain. Purification and characterization. Biochem Pharmacol. (1992) 43: 1975-88.
A purification strategy for inositol 1,4,5-trisphosphate 3-kinase from rat liver based upon heparin interaction chromatography. Cell Signal. (1992) 4: 303-12.
Isolation and characterization of a new Ca2+/calmodulin-dependent protein kinase from isoproterenol-stimulated proliferating rat parotid acinar cells. Biochem Cell Biol. (1992) 70: 250-5.
Isolation and characterization of catalytic and calmodulin-binding domains of Bordetella pertussis adenylate cyclase. Eur J Biochem. (1991) 196: 469-74.
Purification of a soluble isoform of guanylyl cyclase-activating-factor synthase. Proc Natl Acad Sci U S A. (1991) 88: 365-9.
Evidence for a specific recognition site for tiflucarbine on calmodulin. Eur J Pharmacol. (1990) 189: 411-8.
Purification and characterization of a calcium-calmodulin-dependent phospholamban kinase from canine myocardium. Biochemistry. (1989) 28: 5909-16.
The effect of calmodulin on the interaction of carbodiimides with the purified human erythrocyte (Ca2+ + Mg2+)-ATPase. Biochim Biophys Acta. (1988) 945: 33-40.
Purification of D-myo-inositol 1,4,5-trisphosphate 3-kinase from rat brain. J Biol Chem. (1988) 263: 7465-71.
Characterization of the calmodulin-binding sites of muscle phosphofructokinase and comparison with known calmodulin-binding domains. J Biol Chem. (1987) 262: 9454-62.
Isolation and properties of the active gamma subunit of phosphorylase kinase. J Biol Chem. (1986) 261: 4732-7.
Calmodulin activation of cyclic AMP phosphodiesterase in the B16 mouse melanoma. Biochem J. (1984) 219: 941-6.
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Usage : bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product , food additive or as a household chemical.
Hazmat Shipping : Non-hazardous
Storage : 2-8°C
Brand Name : bioPLUS™, Separopore®, ViewIP™
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