Candida sp. PCR Kit CE/IVD
€0.00
In stock
SKU
RealCycler CAND-
Catalog Number: RealCycler CAND-
1 Intended use:
RealCycler CAND is an in vitro diagnostic kit of reagents which allows real-time PCR qualitative detection of DNA from Candida sp. in clinical samples.
The system includes an internal control CHIC (Competitive Heterologous Internal Control) to prevent false negatives due to reaction inhibition.
2 Principle of the test
The polymerase chain reaction (PCR) is based on the amplification of a specific region of the DNA/RNA by using complementary primers to the target sequence. Real-time PCR uses marked probes with fluorophores that emit fluorescence in the case of amplification. The cycle of the PCR protocol in which appears significant fluorescence is proportional to the DNA/RNA quantity present in the sample. This value is called Cycle Threshold (Ct) or Cycle Quantification (Cq).
The amplification of Candida sp.is detected in the corresponding channel of FAM fluorophore and the internal control is detected in the corresponding channel of Alx532 or HEX fluorophore.
3 Technical specifications
Sensitivity: Candida sp.: 100 copies/μL.
The analytical sensitivity has been determined by limit dilution. This sensitivity has been showed in repeated assays with reproducibility over 95%.
Specificity: Candida sp.: Internal Transcribed Spacer Regions.
Specificity validation has been performed according to experimental assays and BLAST analysis (www.ncbi.nlm.nih.gov/blast).
4. Contents
RealCycler CAND includes the AmpliMix and a Candida albicans DNA Positive Control.
All reagents are ready to use without adding or rebuilding any component.
RealCycler CAND is an in vitro diagnostic kit of reagents which allows real-time PCR qualitative detection of DNA from Candida sp. in clinical samples.
The system includes an internal control CHIC (Competitive Heterologous Internal Control) to prevent false negatives due to reaction inhibition.
2 Principle of the test
The polymerase chain reaction (PCR) is based on the amplification of a specific region of the DNA/RNA by using complementary primers to the target sequence. Real-time PCR uses marked probes with fluorophores that emit fluorescence in the case of amplification. The cycle of the PCR protocol in which appears significant fluorescence is proportional to the DNA/RNA quantity present in the sample. This value is called Cycle Threshold (Ct) or Cycle Quantification (Cq).
The amplification of Candida sp.is detected in the corresponding channel of FAM fluorophore and the internal control is detected in the corresponding channel of Alx532 or HEX fluorophore.
3 Technical specifications
Sensitivity: Candida sp.: 100 copies/μL.
The analytical sensitivity has been determined by limit dilution. This sensitivity has been showed in repeated assays with reproducibility over 95%.
Specificity: Candida sp.: Internal Transcribed Spacer Regions.
Specificity validation has been performed according to experimental assays and BLAST analysis (www.ncbi.nlm.nih.gov/blast).
4. Contents
RealCycler CAND includes the AmpliMix and a Candida albicans DNA Positive Control.
All reagents are ready to use without adding or rebuilding any component.
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