Caspase-2 (clone ), anti-human, mouse, rat

Background:
Caspase-2 (Nedd2, ICH-1) is an aspartate-specific cysteine protease that is activated in response to various apoptotic stimuli. Caspase-2 is unique among the caspases in that it has features of both upstream caspases (long prodomain) and downstream caspases (DEXD substrate specificity). Caspase-2 is highly expressed in the brain during development and is expressed at low levels in adult tissue. Specifically, caspase-2 localizes to the mitochondria, the Golgi, the cytoplasm and the nucleus. Caspase-2 exists as two isoforms, caspase-2L and caspase-2S, which are produced by alternative splicing and differ in their N- and C-termini. Caspase-2L acts as a positive regulator of apoptosis, whereas caspase-2S functions as a negative regulator of apoptosis. Following apoptotic stimuli, the caspase-2L precursor undergoes cleavage at Asp 153 to produce a fragment (p30). The p30 fragment undergoes further cleavage to generate a fragment containing amino acids 153-308 (p18) and a fragment containing amino acids 317-435 (p13 or p14). As apoptosis progresses, the p13 (p14) fragment can undergo further processing to yield a fragment containing amino acids 331-435 (p12).

Alternative Name:
Caspase-2, Caspase 2, Caspase2, CASP-2, Neural precursor cell expressed developmentally down-regulated protein 2, NEDD-2, Protease ICH-1, CASP2, ICH1, NEDD2

Application Dilution: WB: 1:1000-1:2000, ICC/IF: 1:50-1:200, IHC: 1:50-1:200, FC: 1:50-1:100

Specificity: This antibody detects endogenous levels of Caspase-2 and does not cross-react with related proteins.

Immunogen:
Recombinant antibody.

MW: ~ 48 kDa

Swis Prot.: P42575

Purification & Purity:
Protein A affinity purified

Format:
Recombinant Rabbit Monoclonal Antibody. 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

For research use only, not for use in diagnostic procedure.