CD10 (CALLA) (clone FR4D11), anti-human

Source:
A BALB/c mice was immunized with Raji cells.
Fusion partner: X63-Ag8.653.



Specifications:
FR4D11 reacts with high affinity to CD10 or CALLA, a cell surface enzyme with neutral metalloendopeptidase activity, inactivating a variety of biologically active peptides. CD10 is a 100 kDa glycoprotein, expressed on 70% of pre-B ALL-cells (common ALL), but also on early lymphoid progenitor-cells in bone marrow and fetal liver. Other normal CD10 positive tissues include renal epithelium, fibroblasts and germinal centre B-cells. Density of CD10 antigen has been shown to be related to cell differentiation and may have prognostic value for B-cell lineage acute leukemia. CD10 is also present on breast myoepithelial cells, bile canaliculi, fibroblasts, with especially high expression on the brush border of kidney and gut epithelial cells.

Species reactivity:
Positive: human.

Applications:
FR4D11 can be used for the classification of acute leukemias and childhood ALL prognosis (patients (CD10+ have a better prognosis than CD10-).
Flow cytometry: +
Frozen sections: +
Immunofluorescence: +

Positive control: Raji cells, tonsil, small intestine or kidney.

References:
- Brown, B. et al., J. Natl. Canc. Inst,.55:1281-1289 (1975).
- Tran-Paterson, R. et al., Blood, 76: 775-782 (1990).
- Doerken, B. et al., in Knapp, W. et. al. (eds)., Leucocyte Typing IV, Oxford Univ. Press, pp 33-34.
- Lavabre-Bertrand, T., et. al., Cytometry, 18: 209-217 (1994).