CD100 (clone 133-1C6), anti-baboon, bovine, human

Source:
A BALB/c mice were immunized with PHA stimulated PBL.
Fusion partner: NS-1.



Specifications:
133-1C6 reacts with human CD100, a 100 kDa homodimer cell-surface antigen that is expressed on resting and PHA-stimulated T cells. It is absent from bone marrow, erythrocytes, eosinophils and endothelial cells. The protein is weakly expressed on NK cells, EBV transformed B cells, monocytes and tumor T cell lines. It plays a role in homotypic cell adhesion and in T cell activation. 133-1C6 was clustered at the IVth WLDA (code A022; Workshop VI code N-L026).

Species reactivity:
Positive: baboon, cow, human. Negative: horse.

Applications:
133-1C6 can be used for demonstration of CD 100 in a variety of applications. CD100 induces B cells to aggregate and improves their viability in vitro.
Flow cytometry: +
Frozen sections: +

Positive control: Daudi, Raji, HUT-78, Kg1a and U937cells, PBL, tonsil and lymph node.

References:
- Knapp, W. et. al. (eds)., Leucocyte Typing IV, Oxford Univ. Press, pp 193-196 (1989).
- Kishimoto T et al. (eds) Leukocyte Typing VI, Garland Publishing, New York, (1997)