CD100 (clone EBS-CD-045), anti-human, mouse, rhesus monkey, cynomolgus monkey

Source:
A BALB/c mouse was immunized with PHA stimulated PBL.
Fusion partner: NS-1.



Specifications:
EBS-CD-045 reacts with human CD100, a 150 kDa homodimer cell-surface antigen that is expressed on resting and PHA-stimulated T-cells. It is absent from bone marrow, erythrocytes, eosinophils and endothelial cells. The protein is weakly expressed on NK-cells, EBV transformed B-cells, monocytes and tumor T-cell lines. It plays a role in homotypic cell adhesion and in T-cell activation.

Species reactivity:
Positive: cynomolgus monkey, human, mouse, rhesus monkey.

Applications:
EBS-CD-045 can be used for demonstration of CD100 in a variety of Applications:
. CD100 induces B-cells to aggregate and improves their viability in vitro.
Flow cytometry: +
Frozen sections: +
Immunofluorescence: +

Positive control: Daudi, Raji, HUT-78, Kg1a and U937 cells, PBL, tonsil and lymph node.

References:
- Hall K, et al. P. Natl. Acad. Sci. USA93: 11780 (1996).
- Mizrahi S, et al. PLoS One.2(9): e818 (2007).
- Yoshino N, et al.. Exp. Anim. (Tokyo)49: 97 (2000).
- Schlossman SL Bloumsell W Gilks et al. eds. (1995). Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press New York.
- Bougeret CIG et al, J. Immunol.148: 318 (1992).
- Knapp WB Dorken EP. Rieber et al, eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press New York