CD106 (V-CAM1) (clone B-K9), anti-human

Source:
A BALB/c mouse was immunized with activated umbilical cord vein endothelial cells (HUVEC).
Fusion partner: X63/AG.8653.



Specifications:
CD106 is a protein of 110 kDa, also known as vascular cell adhesion molecule-1 (VCAM-1) and INCAM-100. CD106 is a member of the Ig superfamily of adhesion molecules and is expressed at high levels on cytokine stimulated vascular endothelial cells, and at minimal levels on un-stimulated endothelial cells. It is also present on follicular dendritic cells of lymph nodes, myoblasts, and some macrophages. In addition, epithelial cells and cancers cells can be positive, like in kidney and prostate cancer. CD106 serves as a ligand for leukocytes integrin (VLA-4 or CD49d/CD29) and mediates cell adhesion of leukocytes to activated endothelium. It plays a role in various immunological and inflammatory responses. B-K9 inhibits the binding of leukocytes to VCAM-1 on stimulated endothelial cells.

Species reactivity:
Positive: human.

Applications:
B-K9 is also used in an immobilized VCAM-1 ELISA.
ELISA: +
Flow cytometry: +
Frozen sections: +
Immunofluorescence: +

Positive control: Human placenta or tonsil, kidney, some prostate cancers.

References:
- Cervelló I et al. PLoS One6:e21221 (2011).
- Lehmann GM et al. Am J Physiol Cell Physiol299: C672-81 (2010).