CD11a (LFA-1) (clone 87-6F9), anti-human

Source:
A BALB/c mouse was immunized with stimulated human leucocytes.
Fusion partner: SP2/0



Specifications:
87-6F9 Reacts with CD11a, a transmembrane molecule with 1145 amino acid residues and a MW of 180 kDa. CD11a is expressed on lymphocytes, granulocytes, monocytes and macrophages. Levels on memory T-cells tend to increase. CD11a plays a key role in mediating leukocyte adhesion to endothelium during inflammatory response through binding to ICAM 1 (CD54). Other ligands are ICAM-2 and ICAM-3. It is also involved in many other T-cell functions and immune phenomena. When paired with CD18, it forms the integrin alphaLbeta2 adhesion. 87-6F9 Potently blocks LFA-1 dependent homotypic cell aggregation and was typed in the IVth International Leucocyte Typing Workshop.

Species reactivity:
Positive: human.

Applications:
87-6F9 Has been tested in flow cytometry and on frozen sections. It can specifically be applied to determine LFA-1 expression on T-lymphocytes and since it blocks LFA-1 dependent homotypic cell aggregation, it can also be used for functional studies.
Flow cytometry: +
Frozen sections: +
Functional studies: +
Immunofluorescence: +
Paraffin sections: -
Western blot: +

Positive control: Human leucocytes.

References:
- Knapp et al. (Eds.), Leukocyte Typing IV, 1989 : sections N1 (pp. 543.551), N1.l (pp. 551-553); N1.2 (pp. 553-554); N1.3 (pp. 555-558); N1.8 (pp. 566-570); N1.9 (pp. 570-574); N14.4 (pp. 689-693).
- Petruzzelli L et al. J Immunol155(2): 854-866 (1995).
- Edwards CP et al. J Biol Chem273(44): 28937-44 (1998).
- Shang XZ et al,. Eur J Immunol28(6): 1970-1979 (1998).
- Tian L et al. J Immunol158(2): 928-936 (1997).