CD11a (LFA-1) (clone CRIS-3), anti-human

Source:
A BALB/c mouse was immunized with stimulated human leucocytes.
Fusion partner: NS-1.



Specifications:
Cris-3 reacts with CD11a, a transmembrane molecule with 1145 amino acid residues and a MW of 180 kDa. CD11a is expressed on lymphocytes, granulocytes, monocytes and macrophages. Levels on memory T-cells ten to increase. CD11a plays a key role in mediating leukocyte adhesion to endothelium during inflammatory responses through binding to ICAM-1 (CD54). Other ligands are ICAM-2 and ICAM-3. It is also involved in many other T-cell functions and immune phenomena. When paired with CD18, it forms the integrin alphaLbeta2 heterodimer also called LFA-1. LFA-1 is chiefly responsible for lymphocyte adhesion. CRIS-3 was typed in the Second International Leucocyte Typing Workshop.

Species reactivity:
Positive: human.

Applications:
CRIS-3 has been tested in flow cytometry and on frozen sections. It can specifically be applied to determine LFA-1 expression on T-lymphocytes.
Flow cytometry: +
Frozen sections: +
Immunofluorescence: +
Paraffin sections: -

Positive control: Human leucocytes.

References:
- Reinherz et. al., EL. Leukocyte Typing II. New York: Springer-Verlag (1985).
- Jain et al. Folia Neuropathol 47(1), 33-42 (2009).