CD11b (MAC-1) (clone EBS-CD-010), anti-human

Source:
A BALB/c mouse was immunized with human neutrophils.
Fusion partner: P3-X63-Ag8.653.



Specifications:
Integrin αM (also designated complement component receptor 3 α-chain; CD11b (p170); macrophage antigen α polypeptide; cell surface glycoprotein Mac-1 α-subunit; CR3 α-chain; MAC1A; MO1A and ITGAM) is a cell adhesion molecule that acts as a receptor for cell surface ligands such as intracellular adhesion molecules (ICAMs) or soluble ligands. Integrins are heterodimeric proteins that contain an α-chain and a β-chain. Integrin αM combines with Integrin β2 (CD18) to form a leukocyte-specific integrin referred to as macrophage receptor-1 (Mac-1) or inactivated-C3b (iC3b) receptor 3 (CR3). Integrin αM-β2 is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles.

Species reactivity:
Positive: human. Negative: mouse.

Applications:
EBS-CD-10 is commonly used as a microglial marker in tissues derived from the nervous system.
Flow cytometry: +
Frozen sections: +
Immunofluorescence: +
Paraffin sections: -

Positive control: Human monocytes and granulocytes. Human lymph node and tonsil.

References:
- Beekhuizen H, et al., J Immunol.145(2):510-8 (1990).
- Argenbright LW et al.,J Leukoc Biol.49(3):253-7 (1991).
- Zhou L, et al. J Biol Chem.269(25):17075-9 (1994).
- Miller LJ, et al. J Immunol.137(9):2891-900(1986).